STAT3与P-STAT3在转基因AD小鼠脑组织中的表达及意义

Expression of STAT3 and P-STAT3 in the brain of a transgenic mouse model of Alzheimer's disease

目的通过检测STAT3及P-STAT3在APPswe/PS△E9双转基因阿尔茨海默病(AD)小鼠脑组织中的表达,探讨其在AD发病过程中的可能作用。方法采用免疫组化法检测APPswe/PS△E9双转基因AD小鼠及对照小鼠脑组织中STAT3和P-STAT3的表达。结果 STAT3和P-STAT3表达于脑组织的不同部位。STAT3在转基因AD小鼠和对照小鼠的大脑皮层、基底前脑、海马及小脑中的阳性表达率分别为93.75%、87.50%,87.50%、43.75%,81.25%、37.50%,62.50%、0.00%,其中差异有统计学意义的是基底前脑、海马及小脑中的表达(P<0.05);P-STAT3在转基因AD小鼠和对照小鼠的大脑皮层、基底前脑、海马及小脑中的阳性表达率分别为0.00%、0.00%,68.75%、0.00%,62.50%、12.50%,43.75%、0.00%,其中差异有统计学意义的是基底前脑、海马及小脑中的表达(P<0.05);且STAT3和P-STAT3呈正相关(P<0.05)。结论 STAT3和P-STAT3在转基因AD小鼠的基底前脑、海马及小脑中存在高表达,其可能参与了AD发病的病理过程。

Objective To detect the expression of signal transducer and activator of transcription 3 （STAT3） and P-STAT3 in the brain of the APPswe/PS△E9 double transgenic mouse model of Alzhaimer＇s disease （AD） and invesitgate their possible role in AD. Methods APPswe/PS △E9 double transgenic mice and control mice were examined for cerebral STAT3 and P-STAT3 expressions using immunothistochemistry. Results STAT3 and P-STAT3 were expressed in the different regions of mouse brain. In the transgenic mice and the control mice, the positivity rates of STAT3 were 93.75%and 87.50%in the cerebral cortex, 87.50% and 43.75% in the basal forebrain, 81.25% and 37.50% in the hippocampus, and 62.50% and 0.00% in the cerebellum, respectively, showing significant differences between the mice in the STAT3 expressions in the basal forebrain, hippocampus and cerebellum （P〈0.05）. The positivity rates of P-STAT3 in the two groups were 0.00%and 0.00%in the cerebral cortex, 68.75%and 0.00% in the basal forebrain, 62.50% and 12.50% in the hippocampus, and 43.75% and 0.00% in the cerebellum, respectively, showing also significant differences in the basal forebrain, hippocampus and cerebellum （P〈0.05）. The expression of STAT3 was positively correlated with that of P-STAT3 in transgenic AD mice （P〈0.05）. Conclusion STAT3 and P-STAT3 are highly expressed in the basal forebrain, hippocampus and cerebellum in transgenic AD mice and may participate in the pathological process of AD.