卵泡抑素样蛋白1在溃疡性结肠炎患者外周血和肠组织中的表达及临床意义

Expression of follistatin-like protein 1 in the peripheral blood and intestinal tissue of patients with ulcerative colitis and its clinical significance

目的检测卵泡抑素样蛋白1（FSTL1）在UC患者外周血和肠黏膜组织中的表达情况，分析其与UC疾病活动性的关系。方法收集2010年10月至2012年6月UC患者60例，收集2012年4月至10月肠镜和病理检查均未见明显黏膜病变者30例为对照。应用ELISA法检测uC组和对照组患者外周血血浆中FSTL1的表达水平，组间比较行t检验。应用免疫组织化学染色方法检测UC组和对照组患者肠黏膜组织标本中FSTL1的表达情况，组间比较行卡方检验。对UC患者进行UC疾病活动指数（DAI）评分，其与血浆FSTL1水平的相关性行Pearson相关分析。结果UC组血浆FST：1表达水平[（14．37±1．80）μg／L]高于对照组[（5．80±0．72）Ug／L]，差异有统计学意义（t=25．01，P〈0．05）。uC患者血浆FSTL1表达水平与UCDAI呈正相关（r=0．814，P〈0．05）。UC组肠黏膜组织中FSTL1的阳性表达率[86．7％（52／60）]高于对照组[46．7％（14／30）]，差异有统计学意义（X^2=52．334，P〈0．05）。结论UC患者FSTL1表达水平上升，并与疾病活动性呈正相关，FSTL1可能在UC发生发展中起一定的作用。

Objective To examine the expression of follistatin-like protein 1 （FSTL1） in the peripheral blood and intestinal mucosa tissues of ulcerative colitis （UC） patients and analyze the correlation between its expression and the activity of UC. Methods From October 2010 to June 2012, sixty patients with UC were collected. From April 2012 to October 2012, thirty individuals without any obvious mucosa lesion under colonoscope and eonfirmed by pathological examination were set as control group. The serum expression level of FSTL1 of both UC group and control group were determined by enzyme linked immunosorbent assay （ELISA）. t-test was performed for comparison between groups. The expression of FSTL1 in the intestinal mucosa of UC group and control group was detected by immunohistochemistry. Chi-square test was used for comparison between groups. The patients with UC were scored with ulcerative colitis disease activity index （UCDAI）. Its correlation with plasma FSTL1 was analyzed by Pearson correlation coefficient. Results The serum expression level of FSTL1 of UC group （（14.37＋1.80） μg/L） was higher than that of control group （（5.80± 0.72）μg/L） and the difference was statistically significant （t= 25. 01, P （0. 05）. The serum expression level of FSTL1 of UC group was positively correlated with UCDAI （r=0. 814, P〈0.05）.The positive expression rate of FSTL1 in the intestinal mucosa tissues of UC group （86.7%, 52/60） was higher than that of control group （46.7%, 14/30） and the difference was statistically significant （X2=52.334,P〈0. 05）. Conclusions The expression of FSTL1 of UC patients increases and is positively correlated with disease activity. FSTL1 may play a role in the development of UC.