前列腺癌中netrin-1/UNC5B的表达及临床意义

Expressions of netrin-1 and UNC5B in prostate cancer and their clinical significance

目的:通过比较不同侵袭能力的前列腺癌细胞netrin-1/UNC5B表达量的差异,为前列腺癌寻找新的生物标志物及治疗手段。方法:选取前列腺癌细胞系DU145、22RV1、PC3、PC3M、RWPE-1,通过RT-PCR、Western印迹检测netrin-1/UNC5B的表达及其差异,并通过免疫荧光检测配体netrin-1及其受体UNC5B在前列腺癌细胞中的定位。结果:netrin-1/UNC5B在前列腺癌细胞中均表达,侵袭能力强的前列腺癌细胞中netrin-1表达量明显增多(P<0.05),而UNC5B在RWPE-1(正常细胞)中表达量明显增多(P<0.05)。结论:netrin-1/UNC5B表达量与前列腺癌的浸润及进展程度紧密关联,有望作为一种潜在的生物标志物来预测前列腺癌的恶性程度。

Objective： To search for a new diagnostic biomarker for prostate cancer by comparing the differences in the expres- sions of netrin-1 and UNCSB in prostate cancer cells with different invasive abilities. Methods： We examined the expressions of ne- trin-1 and UNCSB in five prostate cancer cell lines DU145, 22RV1, PC3, PC3M and RWPE-1 using RT-PCR and Western blot, and positioned the ligands netrin-1 and its receptor UNCSB in the prostate cancer cells by immunoflaorescence. Results： Both netfin-1 and UNCSB were expressed in the prostate cancer cells, and the expression of netrin-1 was significantly increased in highly invasive cells （P 〈 0.05 ）, while that of UNCSB in RWPE-1 （normal） cells （ P 〈 0.05 ）. Conclusion ： The expressions of netfin-1 and UNCSB are closely related to the infiltration and progression of prostate cancer, and expected to be as potential biomarkers for predic- ting the malignancy degree of prostate cancer.