塞来昔布体外抑制三阴性乳腺癌干细胞的研究

Inhibitory Effect of Celecoxib on Triple-negative Breast Cancer Stem Cells in vitro

目的研究选择性COX-2抑制剂塞来昔布对三阴性乳腺癌干细胞体外增殖、凋亡、侵袭和黏附作用的影响,并初步探讨其发生机制。方法 CCK-8法检测细胞增殖活性;AV-PI双染法测定三阴性乳腺癌细胞凋亡率;用细胞黏附实验检测干预后干细胞的黏附抑制情况;Transwell侵袭实验检测塞来昔布对干细胞的侵袭抑制。结果塞来昔布可诱导三阴性乳腺癌干细胞凋亡,实验组与对照组比较差异有统计学意义(P<0.05);不同浓度组间比较以及同一浓度不同时间组间比较差异均有统计学意义(P<0.05)。细胞黏附实验显示,各实验组细胞黏附Matrigel凝胶的能力与对照组比较均明显降低,Transwell实验显示,各实验组侵袭细胞数与对照组相比均明显减少,实验组与对照组之间以及实验组各亚组之间差异均有统计学意义(P<0.05)。结论选择性COX-2抑制剂塞来昔布体外可抑制三阴性乳腺癌干细胞体外增殖、侵袭、黏附和诱导其凋亡,其抑制作用与药物浓度和持续时间有关。

Objective To study the effect of selective COX-2 inhibitor celecoxib on the proliferation, apoptosis, invasion and adhesion of triple negative breast cancer stem ceils, and its mechanism. Methods CCK-8 method was used to detect cell activity. AV-PI double staining method was used to identify cell apoptosis. Cell adhesion experiment was used to test the cell adhesion after treatment. Transwell invasion assay was used to detect the inhibitory effects of celecoxib on stem cell invasiveness. Results Celecoxib could significantly induce the apoptosis of triple- negative breast cancer stem cells（P〈0.05）. The differences among different concentration groups, and different times at the same concentration were statistically significant. Compared with the control group, the adhesion to Matrigel gel in treated groups was significantly suppressed,and the number of invasive cells in treated groups was remarkably decreased in Transwell invasion assay（P〈0.05）. Conclusion Selective COX-2 inhibitor celecoxib could inhibit the proliferation, adhesion and invasiveness and induce the apoptosis of triple-negative breast cancer stem cells in a dose- and time- dependent manner in vitro.