肿瘤干细胞标志物CD133、CD44、SOX2、OCT4、ALDH1在非小细胞肺癌组织中的表达及临床意义

Expression and Significance of Cancer Stem Cell Markers CD133, CD44, SOX2, OCT4 and ALDH1 in Non-small Cell Lung Cancer

目的研究肿瘤干细胞标记物CD133、CD44、SOX2、OCT4、ALDH1在非小细胞肺癌(NSCLC)组织中的表达及临床意义,为探索非小细胞肺癌肿瘤干细胞提供参考。方法采用免疫组织化学方法检测70例NSCLC组织、14例非癌组织中的CD133、CD44、SOX2、OCT4、ALDH1蛋白的表达并对结果进行分析。结果 (1)CD133、CD44、SOX2、OCT4、ALDH1在70例NSCLC组织中的阳性表达率分别为88.57%、98.57%、100%、100%、100%,强阳性表达率分别为48.57%、67.14%、67.14%、31.43%、50%;CD133、C C D 4 4在N S C L C与非癌组织中的表达差异存在统计学意义(P均<0.0001),SOX2、OCT4、ALDH1在NSCLC与非癌组织中的表达差异无统计学意义(P均>0.05)。(2)随着病理级别的升高,CD133、CD44、SOX2、OCT4及ALDH1的表达呈上升趋势,分化越低的NSCLC表达上述指标的概率越高,其中CD133、SOX2、OCT4的表达在高、中、低分化组织中差异存在统计学意义(P值分别为0.001、0.040、<0.0001);CD133的表达在吸烟史、分化程度、淋巴结转移、肿瘤分期四个因素上差异存在统计学意义(P值分别为0.033、0.001、0.033、0.046);CD44与SOX2的表达在年龄上的差异存在统计学意义(P值分别为0.001、0.040)。结论 NSCLC组织中CD133、CD44、SOX2、OCT4、ALDH1阳性率高,CD133、CD44的表达明显高于非癌组织;CD133、SOX2、OCT4与NSCLC的恶性程度有关;CD44与SOX2与年龄因素有关。

Objective To investigate the expression and significance of cancer stem cell markers CD133, CD44, SOX2, OCT4, ALDH1 in non-small cell lung carcinoma（NSCLC） and to look for cancer stem cells of NSCLC.Methods Seventy cases of NSCLC tissues and 14 cases of non cancer tissues were detected by immunohistoehemistry for CD133, CD44, SOX2, OCT4 and ALDH1. Results Seventy NSCLC tissues, The positive expression rates of CD133, CD44, SOX2, OCT4 and ALDH1 were 88.57%, 98.57%, 100%, 100% and 100%,respectively. And strong positive expression rates were 48.57%, 67.14%, 67.14%, 31.43% and 50% respectively. The expression rates of CD133 and CD44 were significantly higher than those in non cancer tissues （P 〈 0.0001） ; The expression rates of SOX2, OCT4 and ALDH1 showed no significant difference between NSCLC and non cancer tissues （P〉0.05）. The expressions of CD133, CD44, SOX2, OCT4 and ALDH1 were increased along with the pathology grades. The expressions of CD133, SOX2 and OCT4 were significantly higher in poor differentiated than those in the well differentiated （p=0.001,0.040 and 〈0.0001）. The expression of CD133 was significant difference （P=0.033, 0.001, 0.033, 0.046, P〈0.05 ）in smoking history, differentiation, lymph node metastasis, staging factors. The expressions of CD44 and SOX2 were significant difference in age （P=-0.001,0.040）. Conclusion The positive expression rates of CD133, CD44, SOX2, OCT4 and ALDH1 were increased,and CD133 and CD44 expressions in NSCLC were significantly higher than those of non cancer tissue. CD133, SOX2 and OCT4 were associated with historical types and CD44 and SOX2 with ages.