缺血预处理对大鼠压疮缺血再灌注损伤保护作用的实验研究

The protective effect of ischemia pre-treatment on pressure ulcer of ischemia reperfusion injury in rats

目的比较不同缺血处理方法对大鼠皮肤及皮下组织的保护作用,为临床判断压疮损伤的程度及压疮干预效果提供理论依据。方法将60只SD大鼠随机分为对照组(S组)、缺血再灌注组(RI组)和缺血预处理组(IPC组),每组20只,建立大鼠压疮缺血再灌注损伤处理模型,通过肉眼观察受压程度,检测血清氧自由基的水平,以判断压疮缺血再灌注损伤程度。结果RI组和IPC组大鼠出现Ⅰ期压疮的发生率为100%。IPC组血清一氧化氮、丙二醛含量高于S组,明显低于RI组,超氧化物歧化酶活性低于S组,明显高于RI组。结论缺血预处理对压疮缺血再灌注损伤具有保护作用。

Objective To compare the protective effect of different pretreatment method on rat skin and subcutaneous tissue in order to provide a theoretical basis for the clinical judgment of the stage of pressure ulcer and its intervention results. Methods 60 rats were randomly divided into three groups including control group （group S）, ischemia-reperfusion group （group RI）, and ischemic pretreatment group （group IPC）, with 20 rats each group. The degree of ischemia reperfusion injury was judged by establishing ischemia pre-treatment mode of rats, observing the stage of pressure ulcer and testing free radical. Results The incidence of pressure ulcer at stage I in group RI and group IPC are 100%. The serum concentration of nitric oxide （NO） and nitric malondialdehyde（MDA） in group IPC were higher than those in group S but lower than those in group R1. The activity of superoxide dismutase （SOD） in group IPC was higher than that in group RI but lower than that in group S. Conclusion The ischemia pre-treatment on pressure ulcer has a protective effect on ischemia reperfusion injury.