深圳地区2003-2012年献血者HBsAg/NAT检测结果情况分析

Analysis of HBsAg / NAT test results of the blood donors in Shenzhen city in 2003-2012

目的分析深圳地区2003-2012年期间献血者HBV感染的流行走势情况。方法所有献血者血样均使用进口与国产酶联免疫试剂,以及血液病毒核酸检测(NAT)方法筛查HBsAg和HBV DNA,采用χ2检验进行统计分析献血者感染HBV的流行趋势。结果深圳地区2003-2012年采血量稳步增长,HBsAg阳性检出率总体呈下降趋势,HBsAg+/NAT+与HBsAg+/NAT-检出率结果相当(1.2∶1);献血者中HBsAg-/HBV DNA+阳性率呈升高趋势。不同年龄组献血者HBV感染率差异无统计学意义(P>0.05)。结论酶联免疫与NAT方法为重要的互补检测手段,能进一步确保输血安全,而隐匿性乙型肝炎病毒感染呈升高态势,应引起高度重视。

Objective To study the trend of HBV infection in blood donors in Shenzhen city in 2003-2012. Methods Blood samples were tested using both domestic and imported ELISA reagents along with nucleic acid detection for HBsAg and NAT. The data were analyzed with X2 test using SPSS software. Results Data showed that with the steady growth in the volume of blood donation, positive detection rate of HBsAg declined each year and the detection rate of HBsAg＋/NAT＋ was basically the same as HBsAg＋/NAT- （ 1.2：1 ） ; But positive detection rate of HBV DNA in HBsAg- blood samples was higher than before. After analyzed by SPSS software, we found that the rate of HBV infection showed no significant difference in gender or age, respectively （P〉0.05）. Conclusion Our experimental results suggested that we had better use both ELISA and nucleic acid testing to ensure the safety of blood transfusion. Besides, we should pay more attention on the sustainable growth incidence of occult hepatitis B infection.