过敏性哮喘大鼠模型结肠和肺组织CCK-8、CGRP、SP及VIP变化观察

Changes of CCK-8,CGRP,SP,and VIP in the Colon and the Lung Tissue of Allergic Asthma Model Rats:an Experimental Observation

目的观察过敏性哮喘大鼠模型消化系统各组织八肽胆囊收缩素(cholecystokinin octapeptide,CCK-8)、降钙素基因相关肽(calcitonin gene related peptide,CGRP)、P物质(substance P,SP)、血管活性肠肽(vasoactive intestinal peptide,VIP)变化。方法以1%卵清蛋白复制肺病(过敏性哮喘)大鼠模型,观察其对从胃至直肠整个消化系统的6个主要部位(胃、十二指肠、空肠、回肠、结肠和直肠)的病理形态和相关调控物质CCK-8、CGRP、SP、VIP的影响。结果模型大鼠肺和结肠组织病理形态同步改变,而胃、十二指肠、空肠、回肠、直肠无明显变化。模型大鼠肺组织和结肠组织CCK-8(79 961.4±12 577.9,48 519.5±12 240.7)、CGRP(41 950.1±12 600.1,38 059.8±11 942.4)、SP(88 243.9±32 177.2,47 417.8±16 462.4)、VIP(20711.4±7 334.6,43 208.1±13 433.8)均出现显著变化(P<0.05,P<0.01),而胃、十二指肠、空肠、回肠和直肠的上述物质无显著变化(P>0.05)。结论肺病可影响到结肠,引起结肠组织出现病理改变和相关调控物质(CCK-8、CGRP、SP、VIP)发生改变;而对胃、十二指肠、空肠、回肠和直肠的影响不显著。

Objective To observe changes of cholecystokinin octapeptide （CCK-8）, calcitonin gene related peptide （CGRP）, substance P （SP）, and vasoactive intestinal peptide （VIP） in each tissue of the digestive system of allergic asthma （AA） model rats. Methods The pulmonary disease （AA） rat model was duplicated by 1% ovalbumin. Its effect on the pathological morphology of the six main parts of the digestive system （stomach, duodenum, jejunum, ileum, colon and rectum） and related regulating factors such as CCK8, CGRP, SP, and VIP were observed. Results The pathological morphology of the lung was synchronously changed as that of the colon of model rats. But there was no obvious change in the stomach, duodenum, jejunum, ileum, or rectum. Significant changes occurred in CCK8 （79 961.4 ± 12577.9,48519.5±12240.7）, CGRP （41 950.1±12600.1,38059.8±11 942.4）, and SP （88 243. 9 ± 32 177.2,47 417.8 ±16 462.4）, and VIP （20 711.4 ±7 334.6,43 208.1 ±13 433.8）of the lung tissue and the colon tissue of model rats （P 〈0.05, P 〈0.01 ）. But there was no significant change in the aforesaid substances of the stomach, duodenum, jejunum, ileum and rectum （P 〉0.05）. Conclusions Pulmona- ry disease might affect the colon, inducing pathological changes of the colon tissue and changes of related regulating factors such as CCK8, CGRP, SP, and VIP. It showed no significant effect on the stomach, duodenum, jejunum, ileum and rectum.