摘要
目的研究裸鼹鼠(NMR)的生化位点特征,初步建立裸鼹鼠生化位点的检测方法。方法以小鼠近交系的生化位点为参照,应用小鼠的检测方法,检测裸鼹鼠的11种同工酶的活性。结果裸鼹鼠的酯酶-1、转铁蛋白为慢带;葡萄糖磷酸异构酶-1、苹果酸酶-1、异柠檬酸脱氢酶-1为中间带;血红蛋白B链、肽酶-3、碱性磷酸酶-1、磷酸葡萄糖变位酶-1、酯酶-3、过氧化氢酶.2为快带。结论7只裸鼹鼠的11个生化位点为一致的条带,无多态性,表现为纯合型。
Objective A elementary detection method on biochemical marker loci was established to study the characteristics of naked mole rat biochemical loci. Methods With inbred mouse as a reference, 11 isozymes of naked mole rat were detected according to the methods for biochemical markers of mice. Results The esterase-1, transferrin of naked mole rat showed slower bands; glucose phosphate isomerase-1, malic enzyme-l, isocitrate dehydrogenase-1 showed intermediate bands; hemoglobin [3 chain, peptidase-3, alkaline phosphatase-1, phosphate glucose mutase-1, esterase-3, hydrogen peroxide enzyme-2 showed faster bands. Conclusion Biochemical locis of 7 naked mole rat performed consistent bands, showed homozygous, no polymorphism.
出处
《实验动物与比较医学》
CAS
2013年第6期477-480,486,共5页
Laboratory Animal and Comparative Medicine
基金
上海市科技发展基金项目(No.12140900200)}=j(No.13140900400)联合资助
关键词
裸鼹鼠
生化位点
遗传检测
Naked mole rat
Biochemical loci of naked mole rat
Genetic monitoring