邻苯二甲酸酯类物质对蚕豆根尖细胞微核率的影响

Effect of phthalate esters on micronuclei rates in vicia faba root tip cells

目的:研究几种邻苯二甲酸酯类物质的遗传毒性。方法:用不同浓度邻苯二甲酸二甲酯、邻苯二甲酸二乙酯、邻苯二甲酸二丙酯、邻苯二甲酸二丁酯和邻苯二甲酸二(2-乙基己基)酯对蚕豆根尖染毒24 h后,观察蚕豆根尖细胞的微核数。结果:邻苯二甲酸二甲酯在浓度为0.008、0.016和0.032 mg/L,邻苯二甲酸二丁酯和邻苯二甲酸二(2-乙基己基)酯在浓度为0.016和0.032mg/L时,所致蚕豆根尖细胞微核率均显著高于阴性对照组(P<0.05)。邻苯二甲酸二乙酯和邻苯二甲酸二丙酯染毒后的蚕豆根尖细胞微核率与阴性对照组比较差异无统计学意义(P>0.05)。结论:在本实验条件下,邻苯二甲酸二甲酯、邻苯二甲酸二丁酯、邻苯二甲酸二(2-乙基己基)酯对蚕豆根尖具有遗传毒性,低浓度邻苯二甲酸二乙酯和邻苯二甲酸二丙酯对蚕豆根尖不具有遗传毒性。

OBJECTIVE：To study the mutagenicity of some kinds of phthalate esters. METHODS：Vicia faba root tips were exposed to different concentrations of phthalate esters including dimethyl phthalate,dibuthyl phthalate,bis（2-ethylhexyl） phthalate,diethyl phthalate and dipropyl phthalate for 24 hours. Number of micronuclei of vicia faba root tips cells was observed. RESULTS：Compared with the negative control group,while dimethyl phthalate at the concentration of 0.008,0.016,0.032 mg/L,phthalate dibutyl and phthalate dioctyl at the concentration of 0.016,0.032 mg/L,respectively, there were significant differences in micronucleus rate （P〈0.05）. Compared with the negative control group,there were no significant differences in micronucleus rate of phthalate diethyl exposured groups and dipropyl phthalate exposured groups （P〉0.05）. CONCLUSION：The present study demonstrats that dimethyl phthalate and dibutyl phthalate,dioctyl phthalate displayed genetic toxicity and low concentration of diethyl phthalate and phthalate dipropyl showed no genotoxicity.