人Boule基因启动子区结合蛋白的生物信息学分析

Bioinformatic Analysis of Human Boule Hromoter Binding Proteins

目的:对精子发生RNA结合蛋白Boule基因启动子区结合蛋白进行生物信息学分析。方法:从基因参考序列数据库获取Boule基因启动子区序列,使用TFSEARCH程序对启动子序列中的转录因子结合位点进行预测。结果:成功获得长度为2 kb的人Boule基因启动子区序列。该启动子区Threshold score≥90的共有60个转录因子结合位点,涉及SOX家族、GATA结合蛋白家族、热休克因子家族、锌指蛋白Kruppel家族、POU家族、runt家族、同源异型框基因家族、TALE类同源结构蛋白家族、转录因子螺旋环螺旋家族、IKAROS家族、FOX家族11个家族的转录因子和3个TATA box。结论:Boule基因表达的调控是在一定时间或空间上、一种或多种调节蛋白作用的复杂过程。调控Boule基因表达的转录因子绝大部分与胚胎发育、性别决定、个体生长密切相关。

Objective： To analyze the human Boule promoter binding proteins using bioinformatics tools. Methods： The sequences of human Boule promoter were obtained from UCSC Genome Bioinformatics. The binding sites of transcription factors were predicted using TFSEARCH software. Results： The sequence Of the promoter of Boule was obtained. And 60 transcription factor binding sites were found within the promoter region （Threshold score -〉 90, involving the SOX, GATA-binding proteins, heat shock factor, zinc finger protein Kruppel, POU, runt, homeobox gene family, TALE class homeodomain protein family, Helix loop helix family of transcription factors, IKAROS, FOX up to 11 families of transcription factors and three TATA boxes. Conclusion： Regulation of the Boule gene expression is a complex process in a certain period of time or space with one or more regulator proteins. The transcription factors regulating Boule gene expression are most related to embryonic development, sex determination, and individual growth closely.