洗涤法与迎头色谱法分离红霉素A和红霉素C

Separation of Erythromycin A and C by Washing Process and Frontal Chromatography

采用大孔树脂SP825分离红霉素A(EA)及其主要杂质红霉素C(EC)。通过平衡试验考察了溶剂极性对SP825吸附EA和EC的影响;以洗涤法和迎头色谱法分离EA和EC,考察洗涤流速、洗涤液pH值对洗涤曲线的影响,并对比了两种分离方式的洗脱产品。结果表明,含3%乙酸乙酯的磷酸盐缓冲液为极性最佳的溶剂;洗涤流速10.5 ml/h、pH 7.0较适宜;洗涤法与迎头色谱法均能实现EA与EC的有效分离,综合考虑成本及分离周期,迎头色谱法优于洗涤法。

Macroporous resin SP825 was adopted to separate erythromycin A （EA） and its main impurity, erythromycin C （EC）. Adsorption equilibrium experiments were carried out to investigate the effect of adjustment of aqueous solvent polarity on adsorption behaviors of EA and EC. Washing process and frontal chromatography were used to separate EA and EC. The effects of washing velocity and pH on washing curves were determined. The elution products of two separation methods were compared. The results showed that phosphate buffer containing 3% acetic ethyl as solvent, washing flow rate of 10.5 ml/h and pH 7.0 were appropriate. Both washing process and frontal chromatography could remove EC efficiently, while frontal chromatography was better for comprehensive consideration.