摘要
目的:通过乙肝病毒感染精子的膜蛋白,研究乙肝病毒(HBV)感染精子的分子机制,并进一步为体外阻遏HBV父婴垂直传播提供理论依据。方法:正常人精液与HBV阳性患者血清及ASGPR Mab共培养,实验分为以下4组:A组,正常人精液;B组,HBV阴性血清与精液标本共培养;C组,HBV阳性血清与人精子共培养;D组,ASGPR MAb和HBV阳性血清与精液共培养。每小时观察记录精子活率(a+b)变化,C、D组24 h标本行流式细胞检测精子膜表面HBsAg、HBcAg。结果:1 h精子活率A>D组、C>B组、C>D组,差异有统计学意义。2 h精液活率A>C组、B>C组、D>C组,差异有统计学意义。3 h精液活动率4组差异均有统计学意义。4~6 h精液活动率除B组与D组无统计学差异外其余各组均有统计学差异。7h活动率A>B组、A>C组、B>C组、D>C组,差异有统计学意义(P值均<0.05)。精子膜表面HBcAg蛋白两组无差异,HBsAg蛋白C组明显高于D组。结论:①ASGPR Mab能有效防止HBV感染精子引起的精液参数的改变。②ASGPR是HBV与精子细胞膜结合的关键蛋白之一,阻遏ASGPR能降低HBV感染人精子。
Objective ： To study the mechanism of HBV infection through detecting HBV （ hepatitis B virus） membrane receptor in sperm, and then, establish the theoretical supports of HBV block in vitro. Methods： Normal semen were cultured with HBV positive serum and ASGPR Mab. Experimental group was divided into the following four groups ： Group A ： normal semen; Group B ： HBV negative serum and normal semen ; Group C ： HBV positive serum and normal semen; Group D ： ASGPR Mab （ asialoglycoprotein receptor monoeionat anti- body） , HBV positive serum and normal semen. Sperm motility （ a ＋ b） were observed and recorded for groups every hour. Samples of group C and D made flow cytometry analysis to detect the HBsAg and HBcAg in sperm membrane surface after 24 - hours. Results ： Sprem live rate afterl hour^cocultured, groupA〉D, C〉B, C〉D （P〈0.05） . 2hoursA〉C, B〉C, D〉C （P〈0.05） . 3 hoursA〉D〉B〉C （P〈0.05） .4-6hoursA 〉 B = D 〉 C （P〈0.05） . 7hoursA〉B, A〉C, B〉C, D〉C （P〈0.05） . There was no difference in group C and D to HBcAg detected, but group C was higher than group D to HBsAg detected in sperm membrane surface. Conclusion： ① ASGPR Mab can effectively prevent the sperm parameters changing caused by HBV. ②ASGPR is one of the most important membrane protein receptor to HBV paternal - fetal vertical transmission, inhibit ASGPR can prevent the process.
出处
《中国妇幼保健》
CAS
北大核心
2013年第36期6007-6010,共4页
Maternal and Child Health Care of China
