玻璃化冷冻人活检后不同发育时期胚胎的研究

Study on vitrification of biopsied human embryos at different stages

目的:探讨玻璃化冷冻法应用于人活检后不同发育时期胚胎冻存的可行性及最佳的冷冻时期。方法:选择体外授精-胚胎移植周期病人授精后第3天的废弃胚胎,随机分为活检组和对照组,活检组胚胎采用抽吸法活检1个卵裂球,对照组胚胎不活检,而后分别继续培养至卵裂期、融合期和囊胚期进行玻璃化冷冻,比较两组各时期胚胎解冻后的复苏效果和继续发育潜力。结果:玻璃化冻融后的活检组卵裂期、融合期和囊胚期胚胎,其解冻复苏率、完整复苏率和继续发育能力均与对照组无显著差异,但活检组卵裂期胚胎卵裂球复苏率显著低于对照组(82.1%vs88.5%,P=0.001);活检组和对照组融合期胚胎完整复苏率均显著高于卵裂期和囊胚期胚胎(P<0.05)。结论:玻璃化冷冻法可以成功应用于人活检后胚胎的冻存,融合期是玻璃化冻融复苏效果最稳定的时期。

Objective： To investigate the feasibility of vitrification on biopsied human embryos at different development stages and the optimal embryo stage for vitrification. Methods ： The discarded embryos on day 3 after fertilization from IVF/ICSI - ET cycles were ran- domly allocated to biopsied and control group, removal of one blastomere by aspiration was performed on embryos fi＇om biopsied group, but the control group not, and then all the embryos were cultured to cleavage, morula and blastocyst stage for vitrification. After warming, the survival efficient and development potential of embryos at different stages from the two groups were compared. Results： After warming, the cleavage, morula and blastocyst stage embryos from both the control and biopsied group had a similar survival rate, intact survival rate and development potential, but the blastomere survival rate of cleavage stage embryos from biopsied group was significantly lower than the control group （82.1% vs 88.5% , P =0. 001 ） . The intact survival rate of the morula stage from both the control and biopsied group was signifi- cantly higher than the cleavage and blastocyst stage embryos （ P 〈 0, 05 ） . Conclusion： Vitrification can be successfully applied for cryopr- eservation of the human biopsied embryos, and the morula stage is the most stable period.