PUMA基因在水飞蓟宾诱导乳腺癌细胞凋亡的作用被引量：2

The role of PUMA gene in apoptosis of breast cancer cells induced by silybin

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摘要目的:探讨凋亡相关基因PUMA在体外水飞蓟宾(silybin,SIL)诱导乳腺癌MCF7细胞凋亡过程中的作用。方法:MCF7细胞分为二组:对照组和SIL组,SIL组处理浓度分别为10、20、40 mg/L,各组细胞分别培养24、48及72 h。MTT法检测SIL对乳腺癌MCF7杀伤率,TUNEL法(DNA断裂的原位末端标记法)测定各组细胞凋亡率,Caspase-Glo⑧3/7定量试剂盒测定细胞Caspase3/7的表达情况,Western blot法检测PUMA蛋白表达水平。结果:上述浓度下的SIL对MCF7细胞增殖均具有抑制作用(P<0.01),其中浓度为40mg/LSIL处理的MCF7细胞在72h时抑制效果最明显,结果呈药物浓度及时间依赖效应;TUNEL法检测显示(48h)SIL可以显著增加MCF7细胞的凋亡率(P<0.01);Caspase3/7检测显示SIL可以使Caspase3/7活性显著升高(P<0.01);Western blot法检测显示SIL可以诱导PUMA蛋白的表达升高。结论:凋亡相关基因PUMA在水飞蓟宾诱导乳腺癌MCF7细胞凋亡过程中具有显著作用,诱导PUMA蛋白表达可能是水飞蓟宾抗癌作用的机制之一。 Objective： To investigate the role of PUMA gene in apoptosis of breast cancer MCF7 cells in vitro induced by silybin （SIL） . Methods： The MCF7 cells were divided into two groups： the control group and the SIL group （ 10, 20, 40 mg/L） , the cells were cultured with SIL for 24, 48 or 72 h. MTT assay was used to detect the survival rate of cells ; TUNEL was used to detect the apoptotic rate of cells ; Caspase - Glo⑧3/7 kits were used to detected the activity of Caspase3/7 ; Western blot method was used to detect the expression of PUMA protein. Results： The MTF assay showed that SIL could inhibited the proliferation of MCF7 cells which depended on dose and time, the effect of 40 mg/L SIL （72 h） was the most obvious; after being treated with SIL （48 h ） , the apoptotic rate of cells and the activity of Caspase3/7 increased significantly ; in addition, PUMA protein increased significantly in SIL -treated group. Conclusion： PUMA may play an important role in the apoptosis of breast cancer MCF7 cells induced by silybin, induced PUMA protein expression may be one of the mech- anisms of anti - cancer effects of silibinin.

作者羊涛张嘉越岑延增李松明李瑞冷津立

机构地区解放军第

出处《中国妇幼保健》 CAS 北大核心 2013年第36期6044-6046,共3页 Maternal and Child Health Care of China

关键词水飞蓟宾 PUMA 乳腺癌 MCF7细胞 Silybin PUMA Breast cancer MCF7 cell

分类号 R937.9 [医药卫生—生药学]

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