牛奶中β-内酰胺类和四环素类药物胶体金快速检测方法的建立

Development of Colloidal Gold Rapid Detection Method of β-lactams and Tetracyclines in Milk

试验旨在采用胶体金免疫层析法检测牛奶中β-内酰胺类药物和四环素类药物残留量。试验制备得到了四环素类药物半抗原，将头孢类药物与四环素类药物半抗原分别与载体蛋白偶联得到免疫原和包被原，用免疫原制备特异性单克隆抗体，建立了一种快速、高通量、简单的检测牛奶中β-内酰胺类药物和四环素类药物残留量的胶体金免疫层析法。利用试纸条同时检测牛奶中12种β-内酰胺类和4种四环素类药物，实现高通量、快速检测，牛奶样品无需稀释，可直接检测，检测时间只需3-5 min，适合中国基层检测实验室和乳品企业大批量样品集中筛查及现场检测。

Colloidal gold immunochromatographic was uesd for the determination of β-lactams and tetracyclines in milk. Tetracyclines hapten was obtained by chemical synthesis. Immunogen and envelope antigen were prepared by cephalosporins and tetracyclines hapten coupled to carrier protein. Monoclonal antibodies were obtained by immunogen immunizing mice. A rapid and simple colloidal gold immunochromatographic assay was established for the determination of β-lactams and tetracyclines in milk. 12 kinds of β-lactams and 4 kinds of tetracyclines were detected at the same time by the strip. The milk sample could be used to be detected without dilution. The detection time could be 3 to 5 min. The method could be efficiently used for mass samples screening in primary laboratory.