茶多酚和L-茶氨酸对热应激下蛋用仔公鸡生长性能及抗氧化能力的影响

Effects of Tea Polyphenol and L-theanine on Growth Performance and Antioxidant Performance of Egg-laying Chickens under Heat Stress

本试验采用单因子试验设计研究茶多酚与L-茶氨酸在热应激下对蛋用仔公鸡生长性能及抗氧化能力的影响。选用1日龄蛋用仔公鸡180只，随机分为6组，每组3个重复，每个重复10只鸡。试验1组为对照组，饲喂基础日粮；试验2-6组分别在基础日粮中添加400 mg/kg茶多酚、200 mg/kg L-茶氨酸、200 mg/kg茶多酚＋200 mg/kg L-茶氨酸、400 mg/kg茶多酚＋200 mg/kg L-茶氨酸、600 mg/kg茶多酚＋200 mg/kg L-茶氨酸，试验期28 d。结果表明，茶多酚可有效降低热应激下蛋用仔公鸡的料重比（P〈0.05），L-茶氨酸单独添加或与茶多酚同时添加对生长性能影响均不显著（P〉0.05）；茶多酚可显著降低各组织器官的丙二醛（MDA）含量 （P〈0.05），显著提高各组织器官的谷胱甘肽过氧化物酶（GSH-Px）与过氧化氢酶（CAT）活性（P〈0.05）；L-茶氨酸能显著提高各组织器官的CAT活性（P〈0.05），不同程度降低各组织器官的MDA含量；茶多酚与L-茶氨酸同时添加时，抗氧化能力与茶多酚的添加量不呈正比；400 mg/kg茶多酚＋200 mg/kg L-茶氨酸组肝脏样总超氧化物歧化酶（T-SOD）与GSH-Px活性均显著降低（P〈0.05），MDA含量显著提高（P〈0.05）。在热应激条件下，200 mg/kg茶多酚＋200 mg/kg L-茶氨酸同时添加时，抗氧化效果较理想。

In order to investigate the effects of tea polyphenols （TP） and L-theanine on growth performance and antioxidant performance of egg-laying chickens under heat stress,single factor design was adopted.180 one-day old chickens were randomly assigned to six groups for three replicates （10 chicks for each）. Group 1 was the control group,was fed on a basal diet;groups 2 to 6 were the treatment groups,were fed with 400 mg/kg TP, 200 mg/kg L-theanine, 200 mg/kg TP and 200 mg/kg L-theanine,400 mg/kg TP and 200 mg/kg L-theanine, 600 mg/kg TP and 200 mg/kg L-theanine in the basal diet. The trial period was 28 d. The results indicated that TP could decrease feed gain significantly （P〈0.05）,L-theanine added alone or combined with TP had no significant effects on growth performance （P〉0.05）. TP could decrease the tissues and organs＇s content of MDA significantly （P〈0.05）,and enhance the activities of GSH-Px and CAT significantly （P〈0.05）.L-theanine could improve the activity of CAT significantly （P〈0.05）,and decreased the tissues and organs＇s content of MDA in different degrees.TP combined with L-theanine, the antioxidant performance was out of step with the addition of TP. When 400 mg/kg TP and 200 mg/kg L-theanine were added,the T-SOD and GSH-Px activities of liver significantly decreased （P〈0.05）,the MDA content increased significantly （P〈0.05）. Under heat stress,the addition of 200 mg/kg TP and 200 mg/kg L-theanine is the most appropriate antioxidant effect.