中草药添加剂对绵羊肌肉组织中鲜味物质含量的影响

Effect of Chinese Herbal Feed Additive on Umami Substances Content in Muscle of Sheep

试验旨在研究自拟中草药添加剂对绵羊肌肉组织中鲜味物质含量的影响。选用32只10月龄绵羊,随机分为4组,即试验1、2、3和4组。试验1组为对照组,饲喂基础日粮;试验2、3和4组在基础日粮中分别添加1%方剂Ⅰ、1%方剂Ⅱ和1%方剂Ⅲ,进行为期60d的饲养试验,采用反相高效液相色谱(RP-HPLC)法检测肉样中肌苷酸(IMP)及其相关代谢物的含量。结果表明,所有试验样品中均不含三磷酸腺苷(ATP)成分,二磷酸腺苷(ADP)、次黄嘌呤(HYP)和一磷酸腺苷(AMP)的含量亦很低,且各组数据间均无显著差异(P>0.05);3个中草药添加剂组均能不同程度的提高绵羊肉中IMP及其分解产物的含量,其中试验4组IMP、肌苷(INO)和校正肌苷酸(IMPc)值均极显著高于试验1组(P<0.01),与试验2、3组相比亦存在显著差异(P<0.05)。提示,方剂Ⅲ能显著改善绵羊肉的风味。

The experiment was conducted to investigate the effect of Chinese herb feed additives on the umami substances content of mutton. 32 healthy 10-month-old Chinese Merino were randomly divided into 4 groups. The group 1 was control group which was given basal diet. The groups 2,3 and 4 were fed the basal diets added with 0.1% Chinese herb feed additiveⅠ,Ⅱ and Ⅲ,respectively. After 60 days feeding,the contents of IMP and its correlative metabolites of samples were determined by RP-HPLC method. The results showed that ATP was not detected in all of the samples. The contents of ADP, HYP and AMP were very low,and there were no significant intergroup differences （P〉0.05）.Three Chinese herb feed additives all could improved the contents of IMP and its correlative metabolites in different degrees. The IMP, INO and IMPc contents of group 4 were extremely significantly higher than those of group 1 （P〈0.01）,and significantly higher than those of groups 2 and 3 （P〈0.05）.The results demonstrated that the Chinese herb feed additive Ⅲ could significantly improve the flavor of mutton.