氟对草鱼肝脏组织显微和超微结构的影响

Effects of NaF on Microstructure and Ultrastructure of the Liver of Grass Carp(Ctenopharyngodon idellus)

试验采用光镜和透射电镜技术,探讨了不同浓度氟化钠对草鱼肝脏组织显微和超微结构的影响。氟化钠暴露浓度分别为0、40、80、120mg/L,每组设2个平行,连续暴露30d,取肝脏组织制备切片,进行光镜和透射电镜观察。结果发现,30d后氟化钠试验组草鱼肝脏组织均有不同程度的损伤,石蜡切片上可观察到试验组草鱼肝细胞索紊乱、断裂,细胞出现空泡变性且病变程度随氟化钠浓度升高而加重,透射电镜下可观察到试验组草鱼肝细胞线粒体轻度肿胀,脊模糊或缺失,线粒体数量减少,胞质中出现空泡。结果表明,氟化钠短期暴露可对草鱼肝脏组织造成损伤,且损伤程度存在剂量依赖效应。

Effects of NaF on microstructure and ultrastructure of the liver of grass carp （Ctenopharyngodon idellus） were estimated by optical and transmission electron microscopy. After exposing to sodium fluoride at the concentrations of 0, 40, 80, 120 mg/L for 30 days, liver tissue sections were prepared for the observation of optical and transmission electron microscopy.The results showed that different degrees of damages were found in liver tissues of grass carp after exposing to different concentrations of NaF for 30 days.The histopathological changes involved disorders and fractures of hepatocyes cord, and vacuolization of liver cells in test groups, which got severer with the increasement of NaF concentrations. Ultrastructural changes showed that mitochondria in liver cells of test groups mild short-term exposed to NaF could cause damages to the liver of grass carp, and the degrees of damage increased with the doses of NaF.