腹腔注射白消安对小鼠生殖能力影响的研究

Study on Effect of Fertility after Mice Treated with Busulfan by Intra-peritoneal Injection

本研究为了探索白消安对小鼠生殖能力的影响，将20只6-7周龄性成熟雄ICR鼠随机分成4组，以注射50%DMSO作为空白对照，分别以每只40、45、50 mg/kg剂量腹腔注射白消安。从注射当天开始分阶段共与母鼠合笼15次，记录了460只合笼母鼠的受孕率、窝产仔数，以及922只后代的性别，并对记录数据进行统计分析，研究了不同剂量药物对小鼠生殖能力的影响。结果表明，腹腔注射白消安能够造成小鼠的短期不育，但这种不育是可以恢复的，且低剂量比高剂量注射恢复更快。白消安虽然会对小鼠的生殖能力造成影响，但不会影响其后代的性别比例。

The effect of fertility after mice treated with busulfan was observed and evaluated in this study. 20 sexually matured male ICR mice with 6 to 7 weeks old were randomly divided into 4 groups. Mice were treated with busulfan in doses of 40,45,50 mg/kg by intra-peritoneal injection, respectively, and perform a blank determination with 50% DMSO. Busulfan-treated mice mated with female 15 times after injection in phases, and we recorded the data of 460 females used for mating, such as conception rate, the litter size and the gender of 922 offspring. Then we measured male fertility after the mice were treated with differential dose by statistical analyses. The short-term infertility result from intra-peritoneal injection of busulfan, which can be recovered by themselves. The recovery of mice fertility treated with low doses was quickly than that with higher doses. The sex ratio of the offspring did not change by the treatment, though it made an impact to fertility.