微囊化与非微囊化胆囊收缩素卵黄抗体对小鼠促生长免疫效果的比较研究

Comparative Study of Microencapsulated and Unmicroencapsulated CCK-IgY on the Growth Immuning Effect in Mice

试验选用70只健康小鼠随机分为7组，每组10只。试验1-7组分别为口服0 mg/kg胆囊收缩素（cholecystokinin，CCK）对照组、口服0 mg/kg空囊组、口服1 mg/kg CCK-卵黄抗体（yolk immunoglobulin，IgY）-低剂量微囊组、口服5 mg/kg CCK-IgY-高剂量微囊组、注射0 mg/kg CCK对照组、注射1 mg/kg CCK-IgY-低剂量非微囊组、注射3 mg/kg CCK-IgY-高剂量非微囊组。单笼饲养，试验期40 d。以小鼠体重、日采食量及血液中CCK抗体D值为评价指标，对CCK-IgY及其微胶囊进行促生长及免疫效果比较研究。结果表明，从试验全期来看，试验3组平均体重和平均日采食量均极显著高于试验4组（P〈0.01），但与试验1组相比差异不显著（P〉0.05）；随着注射CCK-IgY剂量的增加，D值也随之升高，试验7组D值极显著高于试验5组（P〈0.01），而CCK-IgY微囊组均未达到与注射一致的效果。口服微囊化CCK-IgY和腹腔注射非微囊化CCK-IgY均可刺激机体产生相应的免疫应答反应，腹腔注射非微囊化CCK-IgY具有较明显的促生长效果，但CCK-IgY微囊饲喂效果不够明显，需进一步研究。

Seventy healthy mice were randomly divided into 7 groups,each group was 10. The oral feeding 0 mg/kg cholecystokinin （CCK） control group,0 mg/kg empty microcapsules group,1 mg/kg CCK-IgY-LD microcapsules group,5 mg/kg CCK-IgY-HD microcapsules group and injection 0 mg/kg control group,1 mg/kg CCK-IgY-LD injection group, 3 mg/kg CCK-IgY-HD injection group,the experiment period lasted for 40 days. The D value of ovine CCK-IgY,the feed intake and body weight in mice via both forms of treatments were measured, the effect of both forms of treatment was compared each other. The results showed that in whole experimental period,the feed intake and weight gain in group 3 were extremely significantly higher than that in group 4 （P〈0.01）, but it had no significant difference with the group 1 （P〉0.05）. With the increase of CCK-IgY injection dose, the D value was also increased, and the D value of group 7 was extremely sinificantly higher than the control group （P〈0.01）. CCK-IgY microencapsulated group didn＇t had reached the expected effect. Oral feeding and injection of CCK-IgY could stimulate the corresponding immune response in mice. The CCK-IgY injected intra-abdominally to mice presented obvious growth-promoting effect, but the effect of microencapsulated CCK-IgY orally fed to mice showed be further studied.