鼠类感染淋巴细胞脉络丛脑膜炎病毒的RTPCR检测与N基因序列分析

RT-PCR detection and N gene sequence analysis of Lymphocytic choriomeningitis virus in rodents

目的了解宁波市梅山口岸鼠类中淋巴细胞脉络丛脑膜炎病毒（LCMV）流行情况和基因序列特征。方法以LCMVN基因设计2对引物，采用RT—PCR方法对2012年宁波市梅山口岸捕获的鼠类样品进行LCMV检测，并对阳性样品的PCR产物进行测序及同源性和系统进化树分析。结果共检测53份鼠类样品，其中2份小家鼠样品为LCMV核酸阳性，分离的2株LCMV与国外LCMV分离株的同源性在75％~87％之间，与WHI株同源性最高（87％）并在系统发生树上属于同一组。结论证实当地鼠类中存在LCMV流行，新检测到的2株病毒与国外LCMV分离株存在一定差异。

Objective To determine the prevalence and gene sequence of Lymphocytic choriomeningitis virus （LCMV） in the rodents in Ningbo Meishan Port Area. Methods Two pairs of specific primers were designed according to LCMV N gene, and RT-PCR was used to detect LCMV in the rodent samples captured in Ningbo Meishan Port Area in 2012. The PCR products of positive samples were subject to sequencing and then to nucleotide homology and phylogenetic tree analyses. Results Fifty-three rodent samples were tested, and two samples from house mice Mus musculus were LCMV-positive. The two isolated LCMV strains had a homology of 75%-87% with the LCMV strains isolated in other countries; they had the highest homology （87%） with the strain WHI and belong to the same group in the phylogenetic tree as the strain WHI. Conclusion LCMV prevalence in local rodents is confirmed, and there are some genetic differences between the two newly detected LCMV strains and the LCMV strains isolated elsewhere.