浓香型烟叶成熟期质体色素的变化特征研究

Changes of the Plastid Pigment of Tobacco with Great Aroma in Southern Hunan

为探明湖南浓香型烟叶质体色素在生育后期不同阶段和不同叶位间的差异,对云烟87在打顶期、打顶后10 d、打顶后20 d三个时期和上部、中部及下部叶三个叶位间的色素含量变化及叶绿素含量与SPAD值的关系进行了研究。结果表明:同一时期不同叶位叶绿素a、叶绿素b和总叶绿素含量都表现为:上部叶>中部叶>下部叶,除打顶期上部叶和中部叶差异不显著外,不同叶位差异达极显著水平;同一叶位不同时期叶绿素含量变化为,打顶期后下部叶逐步明显减少,中部叶先明显减少后少量增加,上部叶则先增加后降低。不同叶位类胡萝卜素的含量变化在同一时期都表现为:上部叶>中部叶>下部叶,且叶位间存在显著性差异。叶绿素a与叶绿素b含量比值和总叶绿素与类胡萝卜素比值在相同时期都表现为:上部叶>中部叶>下部叶,但前者在打顶期后呈逐渐降低趋势,后者则先增加后明显减少。上部叶、中部叶及下部叶的叶绿素含量均与相应SPAD值具有极显著正相关性。

In order to explore the plastid pigment difference of different periods and different leaf position in the late growth stage of tobacco with great aroma in southern Hunan, the pigments content changes and correlation of chlorophyll content and SPAD value on the upper, middle and lower leaves were studied at topping stage, after topping 10 days and 20 days of YY87. The results showed that the chlorophyll a, chlorophyll b and total chlorophyll contents in different leaf positions at the same period were：upper leaf 〉 middle leaf 〉 lower leaves, except that the difference of upper leaves and middle leaves at topping stage was not significant, differences between different leaf position were extremely significant, and the change trend of chlorophyll content on the same leaf position at different period was：the lower leaves decreased gradually after topping stage, middle leaf raised slightly after the reduction significantly and upper leaves increased first and then de-creased, the change of carotenoid content in different leaf position at the same period was： upper leaf 〉 middle leaf 〉lower leaves, and there was a significant difference in different leaf position;the chl.a /chl.b and the total chlorophyll /carotenoid at the same period showed that：upper leaf〉middle leaf〉lower leaf ,and the former decreased gradually after topping period, while the latter increased first and then decreased. The Chlorophyll content in upper, middle and lower leaves was extremely significant positive correlation with the corresponding SPAD value.