吡格列酮对心肌肥厚大鼠AngⅡ、SOD、NO的影响

Effects of Pioglitazone on AngⅡ、SOD、NO in Cardiac Hypertrophic Rats

目的:探讨吡格列酮对压力超负荷心肌肥厚大鼠血浆中AngⅡ、SOD、NO的影响及可能的作用机制。方法:选用健康雄性SD大鼠40只,随机分为四组:空白对照组、模型组、吡格列酮组和卡托普利组。通过不完全结扎腹主动脉引起压力负荷增加,造成左心室心肌肥厚的模型。术前1周给药,直至术后6周。处死动物,分别测定尾动脉收缩压(SBP),电子天平称量左心室重量(LVW),计算左心系数(LVW/BW);用放射性免疫法检测血浆中AngⅡ,比色法测定血浆中SOD和NO水平。结果:与空白对照组相比,模型组SBP、LVW/BW和血浆中AngⅡ水平明显升高(P<0.05),血浆中SOD和NO水平明显降低(P<0.05);吡格列酮组与模型组相比,血浆中SOD和NO水平明显升高(P<0.05);AngⅡ水平明显降低(P<0.05)。结论:吡格列酮可以通过降低血浆AngⅡ水平,升高SOD和NO水平来抑制压力超负荷增加引起的心肌肥厚。

Objective：To investigate the changes of angiotensin Ⅱ （Ang Ⅱ ）, nitric oxide （NO） and super-oxide dismutase（SOD） level and the possible mechanism, Methods： 40 SD rats were randomly divided into four groups： sham - operated group, model group, pioglitazone - treated group, captopril - treated group. Cardiac hy- pertrophy was produced by abdominal aortic banding. Pioglitazone was given orally 1 week before operation and continued till 6 weeks after operation, then the rats were killed, part of the heart was used for measurements of left ventrieular wall thickness（LVW）, left ventrieular mess index （LVW/BW） and systolic blood pressure（SBP） were measured, Ang Ⅱ level was determined in plasma by radiommunoassay, SOD and NO levels were measured by colorimetric method. Results：As compared with sham- operated group, SBP, LVW/BW and Ang Ⅱ levels of model group in plasma were increased（P 〈 0.05）, SOD and NO levels in plasma were decreased（ P 〈 0.05） ; As compared with model group, SBP, LVW/BW and Ang Ⅱ levels of pioglitazone - treated group in plasma were decreased（ P 〈 0.05 ）, SOD and NO levels in plasma were increased（ P 〈 0.05）. Conclusions： The possible mech- anism of pioglitazone is correlated with inhibiting Ang Ⅱ level and promoting SOD and NO levels in plasma.