新疆光果甘草黄酮对人肝癌Bel-7402细胞增殖的抑制活性研究

Anticancer Activity of Flavonoids from Xinjiang Glycyrrhiza glabra in Human Bel-7402 hepatocarcinoma cell line

目的探讨新疆光果甘草(Glycyrrhiza glabra L)总黄酮及单体成分光甘草定(glabridin,Gb)对人肝癌Bel-7402细胞增殖的抑制活性。方法采用乙醇提取法从光果甘草制备总黄酮提取物(GF-A);经聚酰胺富集法精制而制备黄酮含量高于50%的标准化提取物(GF-B);从GF-B中纯化制备单体成分光甘草定(Gb);以人肝癌Bel-7402细胞株作为体外模型,用MTT法测定各样品对肝癌细胞增殖的抑制活性。结果制备和鉴定3种提取物:GF-A(总黄酮含量31.18%)、GF-B(含量52.5%)和Glabridin单体;3种物质对人肝癌Bel-7402细胞的增殖显示明显的抑制活性,其浓度在25～250μg/mL范围内对肿瘤细胞的最高抑制率分别达67.92%、84.28%和90.11%。结论人肝癌Bel-7402细胞对光果甘草总黄酮的抗癌活性具有一定的敏感性;单体Glabridin可能是光果甘草总黄酮中抗癌活性较高的有效成分之一。

Objective To study the inhibitory activity of flavonoid extracts and monomer glabridin from Xinjiang Glycyrrhiza glabra L （GgL） towards human Bel-7402 hepatocarcinoma cells. Methods General flavonoid extract （GF-A） was prepared from GgL root within ethanol extraction; the extract GF-A was purified within polyamide column chromatography for obtaining fine total flavonoids （GF-B）; monomer glabridin （Gb） was isolated from the extract GF-B; human Bel-7402 hepatocarcinoma cells were respec- tively treated with these samples, and the cell viability was determined by MTT method. Results Three flavonoid samples： GF-A （flavonoid content 31.18~f00）, GF-B （52.5~） and monomer glabridin were char- acterized; these substances showed significant inhibitory activity towards human Bel-7402 cancer cell pro- liferation. At the concentration range of 25 ~ 250 μg/mL, their maximum inhibitory activity was up to 67.92%, 84.28% and 90.11%, respectively. Conclusion The human Bel-7402 hepatocarcinoma cells have certain sensitivity to the anticancer mechanism of flavonoid extracts of GgL; glabridin may be one of the active flavonoid component in this plant with anticancer potency.