宫颈癌人乳头瘤病毒感染与CD4^+CD25^+Foxp3^+调节性T细胞的相关性研究

Correlation of Human Papilloma Virus and CD4^+CD25^+Foxp3^+Treg in cervical cancer

目的探讨宫颈癌人乳头瘤病毒(HPV)感染与T细胞亚群、CD4+CD25+Foxp3+调节性T细胞(Treg)的相关性。方法采用HPV核酸扩增分型检测试剂盒及流式细胞术检测宫颈癌组(40例)、正常对照组(20例)的HPV分型和外周血中T细胞亚群及CD4+CD25+Foxp3+Treg的百分率。结果 HPV阳性的患者外周血T细胞亚群与HPV阴性的患者比较,差异无统计学意义(P>0.05),但调节性T细胞(Treg)在HPV阳性与阴性组之间比较,差异有统计学意义(P<0.05)。宫颈癌组外周血中CD4+CD25+Foxp3+Treg的百分率与正常对照组比较,差异有统计学意义(P<0.05)。HPV与CD4+CD25+Foxp3+Treg细胞呈显著正相关(r=0.613,P<0.05)。结论宫颈癌患者免疫功能与正常人无明显差异,Treg细胞与宫颈癌患者HPV持续感染、肿瘤免疫逃避密切相关。

Objective To investigate the correlation between human papilloma virus （HPV） and T cell subgroup, CD4 ＋ CD25＋ Foxp3＋ T regulatory cell. Methods 40 patients with cervical cancer and 20 normal controls were involved in this study. Typing of HPV was evaluated by Nucleic acid amplification typing kit. The percentage of T cell subgroup, CD4＋ CD25＋ Foxp3＋T cells in peripheral blood was evaluated by flow cytometric analysis. Results T cell subsets showed no statistics difference between HPV negative and HPV positive patients （P ~ 0.05）, but there were significant differences of T regulatory cells in two groups （P ~0.05）. The percentage of CD4＋ CD25＋ Foxp3＋ T cells of CD4＋ T lymphocytes in peripheral blood from patients with cervical cancer were significantly different in comparison with the normal controls （P d0. 05）. CD4＋ CD25＋ Foxp3＋ Treg was positively correlated with HPV （r =0.613 , P 〈0.05）. Conclu- sion There were no significant difference of immunologic function in patients with cervical cancer and health people. Treg is closely related to HPV persistent infection and tumor immune escape in patients with cervical cancer.