Wilson病患者血清氧化物和抗氧化物水平与内脏损伤的关系

The relationship between the levels of serum oxide and antioxide in patients with Wilson disease and its visceral injury

目的 探讨 Wilson病 (WD)患者血清氧化物和抗氧化物水平与内脏损伤的关系。方法 应用化学比色法和反相高效液相色谱法 (RP- HPL C)测定 2 9例 WD患者和 2 1名正常人血清丙二醛 (MDA )、谷胱甘肽过氧化物酶 (GSH- PX)、谷胱甘肽 (GSH)和维生素 E(Vit E)的水平。结果 WD组与对照组相比 MDA显著增高 (P<0 .0 1) ,GSH- PX显著下降 (P<0 .0 1) ,且两者呈负相关 ;血清 Vit E和 GSH在肝型 WD患者显著减少 (分别 P<0 .0 1和 P<0 .0 5 ) ,非肝型患者与对照组比 ,差别无显著性 (P>0 .0 5 )。结论 WD患者血清中存在着自由基系统的代谢紊乱 ,氧化物水平升高 ,抗氧化物水平下降 ,这可能是该病患者内脏损伤的重要原因之一。

Objective To study the relationship between the levels of serum oxide and antioxide in patients with Wilson disease (WD) and its visceral injury.Methods We determined the serum levels of MDA,GSH PX,GSH and VitE in 29 patients with WD and 21 normal controls by the method of reversephase high performance liquid chromatography (RP HPLC) and the chemo colorimetric method.Results The serum levels of MDA in all patients with WD were significantly higher than those of controls( P <0 01),while the serum levels of GSH PX in all patients with WD were significantly lower than those of controls(P< 0 01 );the relationship between MDA and GSH PX was negative correlation.As compared with the controls the serum levels of GSH and VitE were significantly lower in the hepatic type patients with WD(P< 0 01 ,P< 0 05 ),however there was no significant difference between the non hepatic type patients and the controls(P> 0 05 ).Conclusion There was metabolism disorder of free radicals (FRs) in the serum of patients with WD.As compared with normal controls the serum levels of oxides increased and the levels of antioxides decreased in patients with WD,it might be one of important factors for the patients with visce ral injury.