双黄花颗粒镇咳祛痰及免疫作用研究

Study on relieving cough, expelling phlegm, and immunomodulation of Shuanghuanghua Granule

目的观察双黄花颗粒的镇咳、祛痰及调节免疫作用。方法通过小鼠氨水引咳和豚鼠枸橼酸引咳试验,观察双黄花的镇咳作用;通过小鼠酚红排泄试验观察双黄花的祛痰作用;通过小鼠碳粒廓清试验,观察双黄花的调节免疫作用。结果在小鼠氨水引咳试验中,双黄花高、中剂量组能显著延长浓氨水致咳的潜伏期,差异具有统计学意义(P<0.05);双黄花高剂量组能显著减少小鼠浓氨水致咳的次数,差异具有统计学意义(P<0.05);在豚鼠枸橼酸引咳试验中,双黄花高剂量组能显著延长豚鼠枸橼酸引咳的潜伏期和减少咳嗽次数,差异具有统计学意义(P<0.01);在小鼠酚红排泄试验中,双黄花高剂量组能显著增加气管酚红排泄量,差异具有统计学意义(P<0.05);在小鼠碳粒廓清试验中,双黄花高、中剂量组显著提高小鼠廓清指数和吞噬指数,差异具有统计学意义(P<0.05、0.01)。结论双黄花颗粒具有较好的镇咳、祛痰及调节免疫作用。

Objective To evaluate the relieving cough,expelling phlegm,and immunomodulation of Shuanghuanghua Granule（SG）.Methods Cough was induced by ammonia in mice and citric acid in guinea pig.The number of cough was recorded to indicate the effect of SG.The expectorant activities were detected by testing the amount of phlegm secreted in mice.Immunomodulation was evaluated by testing the expectorant carbon clearance in mice.Results Cough was induced by ammonia in mice,high- and mid-dose SG groups could apparently prolong the coughing incubation period（P0.05） and high-dose SG group could apparently reduce coughing frequency（P0.05）;Cough was induced by citric acid in guinea pig,high-dose SG group could apparently increase phenol red excretion in mice（P0.05）;In the expectorant activities experimental,high-dose SG group could apparently increase phenol red sputem excretion in mice（P0.05）;In the expectorant carbon clearance in mice,high- and mid-dose SG groups could apparently decrease the expurgatory index and phagocytic index（P0.01,0.05）.Conclusion SG has the significant effects of relieving cough,expelling phlegm,and immunomodulation.