高转移肺腺癌细胞系A2和A549中p16表达的研究

Research about the Expression of Tumor Suppressor Gene p16 in Two Lung Adenocarcinoma Cell Lines with High Metastasis Potential:A2 and A549

[目的]探讨肿瘤抑制基因对肺腺癌细胞生长的抑制作用。[方法]用FuGene转染方法将p16的表达质粒分别转入两个p16未表达的具高转移能力的肺腺癌细胞系A2和A549中 ,并对转染前后的肿瘤细胞进行了细胞生长曲线、凋亡检测、电镜和流式细胞仪分析。[结果]发现A2、A549细胞系转染后的细胞生长曲线的斜率要比转染前低得多 ,电镜分析也可看出转染前后的细胞形态发生了改变 ,但均未检测到凋亡信号。经流式细胞仪分析发现 :转染后的细胞系均在G0～G1 期发生阻滞。[结论]p16基因在A2、A549细胞中表达可以抑制其生长。对于发生了p16基因突变或缺失而导致p16蛋白在数量或功能上发生改变的恶性肿瘤 。

To investigate the suppression effect of tumor suppressor gene in lung adenocarcinoma cells.We transfected wild type p16 gene into two lung adenocarcinoma cell lines with high metastasis potential:A2 and A549.The suppression effects of p16 gene were evaluated by cell growth curve,apoptosis detection,electron microscope detection and flow cytometric analysis.The gradient of cell growth curve of transfected cells was lower than that of the untransfected.Electron microscopy showed the morphologic change in p16 transfected cells.No detectable apoptosis signal was detected in the transfected cells.The result of flow cytometric analysis suggested that there was delay of these cells in G0～G1 phase after p16 gene transfection.[Conclusion]Our results demostrated that expression of p16 gene can suppress the growth of the tumor cells in A2 and A549.