矽肺患者诱导痰中转化生长因子-β1和肿瘤坏死因子-α的变化

Changes of TGF-β_1 and TNF-α in induced sputum of silicosis sufferers

目的探讨转化生长因子-β1(TGF-β1)和肿瘤坏死因子-α(TNF-α)与矽肺发病的关系。方法采用ELISA法对50名某酒店服务人员的对照人群,50名接触矽尘1年以上的接尘工人,32名矽尘作业观察对象及52例矽肺患者诱导痰中的TGF-β1和TNF-α的含量进行测定。结果与对照组(7.81±2.13)pg/mL比较,接尘组、观察对象组和矽肺组TNF-α水平(49.82±15.42)、(112.13±32.19)、(227.36±52.31)pg/mL明显升高,差异有高度统计学意义(P<0.01);与接尘组(49.82±15.42)pg/mL比较,观察对象组和矽肺组TNF-α(112.13±32.19)、(227.36±52.31)pg/mL水平明显升高,差异有统计学意义(P<0.05,P<0.01)。与对照组(24.12±13.14)pg/mL和接尘组(28.42±15.56)pg/mL比较,观察对象组和矽肺组TGF-β1水平(48.21±16.45)、(61.34±30.22)pg/mL明显升高,差异有统计学意义(P<0.05,P<0.01)。结论 TGF-β1、TNF-α在矽肺发生发展中有重要作用,诱导痰中TGF-β1、TNF-α含量可以反映矽尘所致的肺组织炎症反应。

Objective To investigate the relationship between transforming growth factor-β 1（TGF-β1）,tumor necrosis factor-α（TNF-α） and the incidence of silicosis.Methods Fifty hotel staff were chosen as the control group,50 workers with more than one year of silica dust exposure,32 silica dust observed objects,and 52 silicosis patients as dust exposure group,dust observed group,silicosis patient group respec- tively to detect TGF-β1 and TNF-α levels in their induced sputum with ELISA method. Results Compared with the control group（7.81±2.13）pg/mL,TNF-α（49.82±15.42）,（112.13±32.19）,（227.36±52.31）pg/mL in dust exposure group,observed group and sili- cosis patient group increased significantly,and the difference was of high statistical significance（P ＆lt;0.01）.Compared with the dust exposure group（49.82±15.42）pg/mL,TNF-α（112.13± 32.19）,（227.36±52.31）pg/mL in observed group and silicosis patient group increased signif- icantly,and the difference was of statistical significance（P＆lt;0.05,P＆lt;0.01）.Compared with the control group（24.12±13.14） pg/mL and dust ex- posure group（28.42 ±15.56） pg/mL,TGF-β 1 in observed group and silicosis patient group（48.21 ±16.45）,（61.34 ±30.22） pg/mL increased significantly,and the difference was of statistical significance（P＆lt;0.05,P＆lt;0.01）.Conclusion TGF-β 1 and TNF-α have an important role in the development of silicosis,and the content of TGF-β 1 and TNF-αin induced sputum may reflect the lung tissue inflammation caused by silica dust.