儿童焦虑症与5-HT2A受体基因多态性的关联分析

Associaton analysis between childhood anxiety disorder and 5-HT2A receptor gene

目的 探讨儿童焦虑症与5-HT2A受体基因多态性之间的遗传关联。方法 采用聚合酶链式反应(PCR)和限制性片段长度多态性(RFLPs)方法,对61个符合DSM-Ⅳ与CCMD-2-R诊断标准的焦虑症儿童及其父母的5-HT2A受体基因多态性进行分型,分型结果用单体型相对风险方法(CHRR和HHRR)与传递不平衡检验(TDT)进行分析。结果 儿童焦虑症与5-HT2A受体基因无显著性关联。CHRR、HHRR和TDT值分别为2.3607～0.9213,1.0955和1.88,P值均>0.05。结论 儿童焦虑症与5-HT2A受体基因T102C多态性无关联。

Objectives: To detect the genetic association between childhood anxiety disorder (CAD) and T102C polymorphism in the 5-HT2A receptor gene. Method: The polymorphism of 5-HT2A receptor gene was detected with Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphisms (RFLPs) technique. The association was analyzed with hyplotype relative risk (GHRR and HHRR) and transmission disequilibrium test (TDT) methods in the DSM-Ⅳ and CCMD-2-R diagnosed CAD children (n = 61) and their parents. Results.- No significant association was found between the 5-HT2A receptor gene and CAD (GHRR: x2 = 2. 3607 - 0. 9213, df=i, p>0.05; HHRR: x2 = 1.0955, df = 1, p>0. 05 and TDT: X2 = l.88, df = l, p>0. 05). Conclusion: The results do not support that CAD is associated with the T102C polymorphism of the 5-HT2A receptor gene.