腹部受1150cGy^(60)Coγ线照射小鼠肠道与外周血白细胞的关系

The Relationship between Jejunum and Blood Changing of Mice after Abdominal 1150 cGy^(60) Co γirradiation

[目的 ]观察 115 0 c Gy6 0 Coγ线腹部照射后小鼠的肠道凋亡细胞数、肠腺存活率与外周血白细胞数的变化 ,探讨辐射损伤肠道与外周血白细胞的关系。 [方法 ]选用 70只 BAL B/c雄性小鼠 ,用 115 0 c Gy6 0 Coγ线进行腹部照射 ,随机分为 7组 ,于照射后的第 6、12、2 4、48、72、96、192 h分别进行外周血象、空肠肠腺内的凋亡细胞数和肠腺存活率测定。 [结果 ]照射后随时间延长外周血白细胞、肠腺内的凋亡细胞数、肠腺存活率呈指数函数减少 ,6～ 96 h肠腺内的凋亡细胞、肠腺存活率与外周血白细胞呈直线相关。[结论 ]115 0 c Gy6 0 Coγ线腹部照射后小鼠的肠道变化 。

Objective] To explore relation of the blood hemogram and intestinal changing, three indicators (the apoptosis on the jejunum ,the crypt survival of jejunum and the peripheral blood) were studied in mice after irradiation [Methods] The abdominal area of the 70 BALB/c male mice was irradiated with 1150 cGy 60 Coγ ray Three indicators were counted on 6 ,12 ,24 ,48 ,72 ,96 and 192 h after irradiation [Results] Apoptosis, the crypt survival of jejunum and leucocyte decreased on 6 h, 12 h, 24 h, 48 h, 72 h and 96 h by exponential function The trends between 6 96 h existed linear correlation [Conclusion] The damage of jejunum induced by 1150 cGy 60 Coγ ray could be observed by the changes of leucocyte in peripheral blood