绿原酸纳米脂质体的制备及其质量评价

Preparation of Chlorogenic Acid Nanoliposome and its Quality Evaluation

采用薄膜-均质法制备绿原酸纳米脂质体,并以脂质体包封率为主要考察指标。通过单因素和响应面实验确定了最佳工艺参数:卵磷脂和胆固醇比为5∶1(质量比),卵磷脂和绿原酸比为8.3∶1(质量比),均质压力为62MPa。在此条件下,绿原酸脂质体的包封率为76.32%,平均粒径为278.1 nm,电位为-32.6mV。此法制备的绿原酸脂质体具有包封率高、粒径分布均匀、稳定性好等优点。

The membrane-homogenization method was used to prepare chlorogenic acid nanoliposome.and the encapsulation efficiency was taken as index.The optimum conditions was determined by the One-factor and response surface tests;the phosphatidylcholine ∶ cholesterol was 5 ∶ 1,the phosphatidylcholine∶chlorogenic acid was 8.3 ∶1,the homogeneous pressure was 62 MPa.Under the optimum conditions,the encapsulation efficienc was 76.32 %,the mean diameter of particles was 278.1 nm,the ZETA potential was-32.6 mV.The chlorogenic acid nanoliposome prepared by membrane homogenization method had high encapsulation efficiency,good particle size distribution and stability.