一种乳品中阪崎肠杆菌的快速检验方法

A Method of Rapid Determination for Enterobacter sakazakii in Dairy

建立一种快速检验乳品中阪崎肠杆菌的方法。用标准菌株验证快速检验方法的特异性、灵敏性和重复性,与国标、行标检验方法进行比较,阪崎肠杆菌快速检验方法特异性较好,检验灵敏度高,可检出小于1 CFU/100 g的菌落浓度,快速检验方法与国标方法和行业标准方法相比,具有较好的的选择性和特异性,检测效果相当。这种全新的快速检验方法的能缩短检验周期短,价格便宜,具有较好的应用价值。与国家标准方法相比,无显著性差异。

To establish a method of rapid determination for Enterobacter sakazakii in dairy.Verifying the specificity and sensitivity and repeatability of the rapid determination by detecting the standards strains,and comparing the rapid determination with the national standards and Industry standards.the whole detection process takes 24 h-30 h,and the sensitivity is lower than 1 CFU/100 g,by which Enterobacter sakazakii can be effective selected from other microbe.Comparing to theGB and SN methods,it is a simple,quick,accurate,and the detection result is no marked difference.The new method of rapid determination for Enterobacter sakazakii were invaluable which may shorten the detction periods,cheap.