DOG1、CD117及CD34在胃肠道间质瘤中的表达及病理诊断的意义

Expression and diagnostic significance of DOG1, CD117 and CD34 in gastrointestinal stromal tumors

目的探讨DOG1、CD117及CD34在胃肠道间质瘤中的表达及病理诊断的意义。方法应用免疫组化EnVision二步法，检测DOG1、CD117及CD34在60例胃肠道间质瘤（GIST）和14例其他类型间叶源性肿瘤组织中的表达。结果GIST组织中DOG1蛋白表达明显高于非GIST间叶源性肿瘤（P=0．000）。发生于胃的GSIT组织中DOG1蛋白表达明显高于肠道GIST（P=0．011）。60例GIST组织中，DOG1、CD117和CD34蛋白阳性表达率分别为95％、95％和83．3％，DOG1蛋白表达高于CD34（P=0．040），而DOG1与CD117差异无统计学意义（P=1．000）。CD117、CD34阳性表达GIST组织中DOG1表达率分别为94．7％、94．1％，CD117、CD34阴性表达GIST病例均全部表达DOG1（3／3，9／9），其中有3例CD117及CD34两者阴性，但DOG1阳性。结论DOG1是一个诊断GIST具有较高敏感性和特异性的GIST标记物，在GIST临床病理诊断工作中，联合检测DOG1、CD117及CD34蛋白表达情况，有助于进～步提高诊断GIST的准确性。

Objective To investigate the expression and diagnostic significance of DOG1, CDll7 and CD34 in gastrointestinal stromal tumors. Methods Expressions of DOG1, CD117 and CD34 were de- termined by immunohistochemical techniques （ EnVision ） in 60 cases of gastrointestinal stromal tumors （GIST） and 14 cases of other mesenchymal tumors. Results Expression of DOG1 in GIST was significant- ly higher than in other mesenchymal tumors （ P = 0. 000）. Expression of DOG1 in GIST from stomach was significantly higher than that from intestines. The expression rates of DOG1, CD117 and CD34 in GIST were 95% , 95% , and 83.3%, respectively. Expression of DOG1 was significantly higher than CD34 （ P = 0. 040）, but no significant difference between DOG1 and CDll7 （ P =1. 000）. The expression rates of DOG1 in CD117-positive, CD34-positive tumors were 94. 7% and 94. 1%, respectively. All the CDllT- negative,CD34-negative tumors expressed DOG1,3 cases which were negative for CDll7 and CD34 ex- pressed DOG1. Conclusions DOG1 is a novel marker for GIST, It is necessary to the combined detection of the expression of DOG1, CD117 and CD34, which will further improve the diagnostic accuracy of GIST.