野菊花不同萃取部位对肝损伤小鼠肝细胞蛋白合成及小鼠免疫功能的影响

Effects of different extracts of Flos Chrysanthemi indici on protein synthesis capacity in mice with liver injury and immunoregulation in normal mice

目的:观察野菊花不同萃取部位(FCI-A、FCI-B和FCI-C)对肝损伤小鼠肝细胞蛋白合成及小鼠免疫功能的影响。方法:96只小鼠分为12组(每组8只),即正常组,模型组,阳性对照组(BP组)及FCI-A、FCI-B和FCI-C的低、中、高剂量组,灌胃给药7 d后,除正常组外,余11组均给予D-氨基半乳糖制备小鼠肝损伤模型,24 h后眼眶取血测定血清总蛋白(TP)、白蛋白(ALB)水平和谷丙转氨酶(ALT)活性,同时计算肝、脾指数;另取小鼠,常规方法测定野菊花不同萃取部位对小鼠固有免疫功能的影响。结果:模型组小鼠肝、脾指数较正常组升高,而FCI各给药组小鼠肝、脾指数不同程度地降低,其中FCI-A、FCI-B高剂量组小鼠肝、脾指数明显降低,而FCI-C组小鼠脾指数明显降低,且趋于正常(P<0.05)。模型组小鼠血清TP、ALB水平较正常组降低,ALT活性升高,而FCI-A和FCI-B组小鼠血清TP、ALB水平较模型组升高,以FCI-B组更明显(P<0.05)。FCI-B、FCI-C低剂量组小鼠固有免疫功能增强,高剂量组则表现为免疫抑制(P<0.05),FCI-A对免疫功能的影响不明显。结论:FCI-A、FCI-B及FCI-C对肝损伤小鼠肝组织有保护作用,其中FCI-A尤其是FCI-B可提高小鼠肝细胞蛋白合成功能;FCI-B、FCI-C具有免疫调节作用。

Aim：To investigate the effects of Flos Chrysanthemi indici different extracts（ FCI-A,FCI-B,and FCI-C）on protein synthesis capacity in mice with liver injury and immunoregulation in normal mice. Methods：A total of 96 mice were allocated into 12 groups（ 8 in each group） ：normal group,model group,positive control group,FCI-A,FCI-B,and FCI-C low-,mediate-,and high-dose groups,and the mice were given according treatment by gavage; after 7 days,the mice in the 11 groups except for normal group were treated with D-galactosamine to establish the liver injury model. After24 h,the blood samples from the eye vein were obtained to determine serum total protein（ TP）,albumin（ ALB） levels and alanine aminotransferase（ ALT） activity,at the same time,the liver and the spleen were removed and the liver index and spleen index were calculated. The immunoregulation effects of FCI-A,FCI-B,and FCI-C on the mice were detected by regular methods. Results：Compared with normal group,the liver index and spleen index in the model group were increased and those in the FCI-A,FCI-B,and FCI-C groups were decreased especially in the high-dose groups of FCI-A and FCI-B,and the spleen index in FCI-C groups decreased significantly,near to normal level（ all P 0. 05）. Compared with normal group,the levels of TP and ALB in the model group decreased and the activity of ALT increased while the levels of TP and ALB in FCI-A and FCI-B groups increased especially in the FCI-B groups（ all P 0. 05）. FCI-B and FCI-C had immunoregulation function in mice. Conclusion：FCI-A,FCI-B,and FCI-C have protective effects on the liver injury of the mice,among which,FCI-A and FCI-B could increase the protein synthesis capacity; FCI-B and FCI-C could exert regulation effects on the immune system.