自发性高血压大鼠学习记忆能力和脑组织SK2蛋白的表达

Expression of SK protein in brain tissue and the learning and memory capacity of spontaneously hypertensive rat

目的:探讨高血压对学习记忆能力的影响。方法:取自发性高血压(SH)大鼠和对照Wistar-Kyoto大鼠各7只,采用标准Ⅱ导联记录心电图,尾套法测定鼠尾动脉压,采用Y型迷宫和跳台实验测试大鼠的学习记忆能力,采用Western blot法检测脑组织小电导钙激活钾通道蛋白2(SK2)的表达。结果:与对照比较,SH大鼠鼠尾动脉压增高(t=12.060,P<0.001),QRS时长和R-R间期延长(t=3.147,3.917,P<0.05),Y型迷宫实验学习次数减少(t=3.279,P=0.007),跳台实验上台次数和上台受电击总时间增加(t=4.626,2.516,P<0.05);脑组织SK2蛋白表达也较对照组增加。结论:SK通道可能参与了高血压大鼠认知损害的过程。

Aim：To explore the influence of hypertension on learning and memory capacity. Methods：A total of 7spontaneously hypertensive rats were used to detect arterial pressure with tail-trussed method,ECG was recorded using standard lead Ⅱ,and the learning and memory capacity was measured using Y-type electric maze and the jumping stand measurements,and Western blot was used to determine the expression of SK2 protein in brain tissue. Wistar-Kyoto rats were the control. Results：Compared with those of the control,arterial pressure of spontaneously hypertensive rats was higher（ t = 12. 060,P 0. 001）,QRS duration and R-R interval prolonged（ t = 3. 147,3. 917,P 0. 05）,the learning times during Y-type electric maze measurements was less（ t = 3. 279,P = 0. 007）,the times of jumping onto the platform and the total time of shock increased（ t = 4. 626,2. 516,P 0. 05）,and the expression of SK2 protein in brain tissue was higher.Conclusion：SK channel may be associated with learning and memory damages of spontaneously hypertensive rats.