饮水氟暴露对成年男性血清睾酮及雄激素结合蛋白的影响

Influences of fluoride exposure in drinking water on serum androgen binding protein and testosterone of male adults

目的：探讨男性睾酮（T）及雄激素结合蛋白（ABP）与饮水氟暴露的关系。方法：应用现况调查研究,在河南省某县随机选择7个村庄作为调查点,分别为高氟村2个、改水村2个和对照村3个;采集各调查点生活饮用水。整群抽取所选调查点本地出生的18~50岁男性作为调查对象,分别采集晨尿和空腹静脉血。应用氟离子选择电极法测定饮用水和尿中氟含量,采用ELISA法测定血清ABP水平,采用化学发光免疫分析法测定血清T。结果：高氟组饮水氟浓度为（2.44±1.88）mg/L,高于对照组的（0.37±0.15）mg/L和改水组的（0.36±0.30）mg/L（F=12.289,P〈0.001）。高氟组尿氟浓度为（2.49±1.40）mg/L,高于对照组的（1.04±0.49）mg/L及改水组的（1.38±0.67）mg/L（F=71.563,P〈0.001）,改水组亦高于对照组（P〈0.05）。高氟组血清ABP含量为（16.01±10.83）nmol/L,低于对照组的（27.94±31.90）nmol/L及改水组的（22.42±28.12）nmol/L（F=28.807,P〈0.001）。对照组、改水组、高氟组血清T含量为（4.31±1.30）、（4.42±1.37）和（4.74±2.17）nmol/L,差异无统计学意义（F=0.268,P=0.765）。在对照组和改水组中,血清T含量与年龄呈负相关（β=-0.238、-0.262,P均〈0.05）。结论：环境氟暴露可影响男性血清ABP水平。

Aim：To explore the relationship between environmental fluoride exposure and the concentration of serum androgen binding protein（ ABP） as well as testosterone（ T） of male adults. Methods：A cross-sectional study was conducted in 7 villages of Tongxu county including 2 endemic fluorosis villages（ EFV）,2 EFV implemented with defluoridation project villages（ DFPV） and 3 control villages（ CV）. Males who aged 18 to 50 years born in the villages were recruited using cluster sampling. Drinking water,urine and blood samples were collected. The fluoride levels of drinking water and urine samples were detected by fluoride-ion selective electrode method. ABP was determined using ELISA method and T was determined using chemiluminescence method. Results：The fluoride concentrations in drinking water were（ 2. 44 ±1. 88）,（ 0. 36 ± 0. 30） and（ 0. 37 ± 0. 15） mg / L in EFV,DFPV and CV,respectively（ F = 12. 289,P 0. 001）. It was significantly higher in EFV than those in DFPV and CV（ P 0. 05）. Compared with DFPV[（ 1. 38 ± 0. 67） mg / L]and CV[（ 1. 04 ±0. 49） mg/L],the urinary fluoride concentration of EFV[（ 2. 49 ±1. 40） mg/L]was higher（ F =71. 563,P 0. 001）,and there was a significant difference between DFPV and CV（ P 0. 05）. Serum ABP level of adults from EFV[（ 16. 01 ±10. 83） nmol/L]was significantly lower than that from DFPV[（ 22. 42 ±28. 12） nmol/L]and CV[（ 27. 94 ±31. 90） nmol / L]（ F = 28. 807,P 0. 001）. No significant differences were observed in serum T concentration among three groups（ F = 0. 268,P = 0. 765）. There was a negative relationship between T and age of men who were investigated in CV and DFPV（ β =- 0. 238,- 0. 262,P 0. 05）. Conclusion：Environmental fluoride exposure may affect serum ABP level in male.