脂肪来源干细胞复合不同生物支架在裸鼠皮下的促血管化作用

Promoting neovascularization of adipose tissue derived stem cells compounding with different cellular scaffold in nude mice subcutaneous stratum

目的:探讨脂肪来源干细胞复合不同生物支架在裸鼠皮下的促血管化作用。方法:根据所选支架的不同,将实验分为4组,A组为脂肪颗粒,B组为透明质酸钠,C组为Ⅰ型胶原支架,分别按体积比1∶1与25×106mL-1的脂肪来源干细胞混合,D组为单纯脂肪来源干细胞,将4组试剂注射到12只裸鼠皮下构建软组织,每只裸鼠各注射4个点,3个月后获取相应标本,测定湿质量、存活率、微血管密度。结果:4组间湿质量、存活率、微血管密度比较,差异均有统计学意义(F=47.523、48.425和47.619,P均<0.05),且A、B、C组湿质量、存活率、微血管密度均高于D组(P均<0.05)。结论:脂肪来源干细胞复合生物支架可加速软组织血管化,且效果优于单纯脂肪来源干细胞。

Aim：To discuss the affection of promoting neovascularization of adipose tissue derived stem cells（ ADSCs）compounding with different cellular scaffold in nude mice subcutaneous stratum. Methods：The experiment was divided into four groups depending on the selected scaffold. Group A,B and C were given fat particles,sodium hyaluronate,type Ⅰcollagen,respectively,mixed with ADSCs at 25 × 106mL- 1. Group D was given pure ADSCs. Four reagents were injected randomly into subcutaneous stratum of 12 nude mice to build soft tissue. Each nude muse was injected 4 points. The appropriate specimens were obtained three months later and wet weight,survival rate and microvessel density were detected. Results：The differences were statistically significant regarding the wet weight,survival rate,microvessel density among the four groups（ F = 47. 523,48. 425,47. 619,P 0. 05）. The wet weight,survival rate,and microvessel density of group A,B and C were higher than those of group D（ P 0. 05）. Conclusion：ADSCs compounding with cellular scaffold could promote neovascularization in subcutaneous stratum,and is better than pure ADSCs.