花斑癣患者血清中抗糠秕马拉色菌IgG、IgM、IgA抗体的检测及其意义

Detection of antibodies for IgG, IgM and IgA classes against whole Malassezia furfur in sear from patients with pityriasis versicolor and its significance

目的：探讨机体体液免疫在花斑癣发病中的作用和意义。方法：以糠秕马拉色菌（M．furfur）整菌（WMF）为抗原，用间接酶联免疫吸附试验（ELISA）方法，检测68例花斑癣患者和41例正常人血清中的抗WMF抗体。结果：正常人血清中存在高滴度的抗WMF抗体，花斑癣患者血清中抗WMFIgG抗体明显低于正常对照组（P＜0．01），男性患者血清中IgG抗体低于女性患者（P＜0．01），病程1年以上者血清中特异的IgG抗体低于病程不到1年者（P＜0．01）。结论：机体血清抗M．furfur抗体可能是人体内天然抗体，且特异的IgG抗体具有保护作用。支持花斑癣的发病与免疫缺陷有关。

Objectives:To invesgate the role of humoral immunity in the pathgenesis of pityriasis versicolor and its significance.Methods: The serum level of antibodies for IgG, IgM, IgA classes against Malassezia furfur in 68 patients with pityriasis versicolor and 41 normal people was detected by enzyme - linked immunosorbent assay (ELISA)using whole cells of Malassezia furfur (WMF) as antigen. Results: The titre of antibodies to WMF in sera of normal people was higher. The results demonstrated that the serum level of IgG against WMF in patients with pityriasis versicolor, male patients and patients with a disease duration more than one year was significantly lower than that in controls (P < 0.01),female cases (P < 0.01) and patients with a disease duration less than one year (P <0.01) respectively. Conclusions:The antiodes to WMF may be naural antibodies in human body and the IgG ntibody against WMF in serum may have protection from pityriasis versicolor. The results showed that immunity defect is involved in the pathogenesis of pityriasis versicolor.