养阴益气活血方药对干燥综合征NOD小鼠颌下腺Fas/FasL及其mRNA表达的影响

Effect of nourishing Yin,strengthening Qi and activating blood decoction on Fas /FasL in salivary glands of NOD mice with sjogren's syndrome and their mRNA expression

目的:观察养阴益气活血方药对干燥综合征NOD小鼠颌下腺组织Fas/FasL及其mRNA表达的影响。方法:32只NOD小鼠随机分为模型组、中药组(每天按100 g·kg-1灌胃养阴益气活血煎剂0.4 mL)、羟氯喹组(每天按60 mg·kg-1灌胃羟氯喹0.4 mL)、中西药组(每天灌胃养阴益气活血煎剂50 g·kg-1+羟氯喹60 mg·kg-1,0.4 mL),每组8只,选8只Balb/C小鼠做为正常对照组。实验进行8周后处死小鼠,解剖摘取颌下腺,免疫组织化学法检测颌下腺组织Fas,FasL蛋白的表达;RT-PCR法检测颌下腺组织Fas,FasL mRNA的表达。结果:模型组小鼠颌下腺组织Fas,FasL表达均高于其他各组(P<0.05)。对照组FasL表达明显低于羟氯喹组(P<0.05)。模型组小鼠颌下腺Fas mRNA相对表达量均高于其他组,对照组明显低于其他各组(P<0.05)。模型组小鼠颌下腺FasL mRNA表达高于中药组、中西药组(P<0.05);中西药组表达明显低于羟氯喹组(P<0.05)。结论:养阴益气活血煎剂能下调干燥综合征NOD小鼠颌下腺Fas,FasL及其mRNA的表达,且与羟氯喹合用效果较好。养阴益气活血煎剂可能通过减少Fas/FasL调控的细胞凋亡达到治疗SS的作用。

Objective ： To observe the effect of nourishing Yin, strengthening Qi and activating blood decoction on Fas/FasL in salivary glands of NOD mice with Sjogren＇s syndrome and their mRNA expression. Method： Thirty-two NOD mice were randomly di- vided into the model group, the traditional Chinese medicine group （TCM group, orally given O. 4 mL nourishing Yin, strengthening Qi and activating blood decoction as per 100 g · kg-1 everyday）, the hydroxychloroquine group （given 0. 4 mL hydroxychloroquine as per 60 mg · kg-1 everyday）, the traditional Chinese medicine and western medicine group （TCM WM group, given nourishing Yin, Strengthening Qi and activating blood decoction 50 g · kg-land hydroxychloroquine 60 mg · kg-i, 0.4 mL everyday）, with eight mice in each group. Eight Balb/C mice were selected as the normal control group （ normal group）. All of mice were killed after eight weeks, and their submaxillary glands were dissected. The expression levels of Fas/FasL were examined by immunohistochemical method, and the FasL mRNA was detected by RT-PCR. Result： The expression levels of Fas/FasL in salivary glands of the model group were higher than that of other groups （ P 〈 O. 05 ）. The expression level of FasL of the normal group was much lower than that in the hydroxychloro- quine group （ P 〈 O. 05 ）. The relative expression level of Fas mRNA in salivary glands of the model group was higher than that in other groups, but the control group was notably lower than other groups （P 〈 0.05 ）. The expression level of FasL mRNA in salivary glands of the model group was higher than that in TCM and TCM WM groups （P 〈 0. 05）. But the expression level in TCM WM group was no- tably lower than the hydroxychloroquine group （ P 〈 O. 05 ）. Conclusion： The nourishing Yin, strengthening Qi and activating blood decoction could down-regulate the expression level of Fas/FasL in salivary glands of NOD mice with Sjogren＇s syndrome and their mRNA expression, and had a better efficacy after being combined with hydroxychloroquine. The nourishing Yin, strengthening Qi and activating blood decoction might treat the Sjogren＇s Syndrome by reducing apoptosis which is regulated by Fas/FasL.