大豆子叶节再生体系的建立

Establishment of the Cotyledon Node Regeneration System

以吉农28、吉农27、吉农17三个基因型大豆子叶节作为外植体,采用正交实验设计方法,对大豆的基因型和子叶节再生中植物生长调节剂浓度进行了研究。结果表明:适合大豆子叶节不定芽诱导的基因型是吉农27,最佳诱导培养基是MS+6-BA 3.0mg/L+IBA 0mg/L+2,4-D0mg/L。适合大豆子叶节不定芽继代的基因型是吉农17,最佳继代培养基是MS+6-BA 1.0mg/L+IBA 0.1mg/L。适合大豆子叶节不定芽增殖的最佳基因型是吉农27,最佳增殖培养基是MS+6-BA1.0mg/L+NAA 0.1mg/L+GA31.0mg/L。

Using Jinong 28, Jinong27 ,Jinong 17 three genotypes of soybean cotyledon node as explants , using an experimental design, plant growth regulator concentration from the soybean cotyledonary node regeneration was stud- ied. The results showed that ： the best cotyledon node adventitious buds genotype was Jinong B, the best medium was MS ＋6-BA 3.0mg/L ＋ IBA 0mg/L ＋2,4-D 0mg/L. The best soybean cotyledon node adventitious buds subculture genotype was Jinong 17, the best medium was MS ＋ 6-BA 1.0mg/L ＋ IBA 0.1 mg/L. Suitable for the soybean coty- ledon node proliferation of adventitious shoots genotype was Jinong B, the best medium was MS ＋ 6-BA 1. 0mg/L ＋ NAA 0.1 mg/L ＋ GA3 1.0mg/L.