杜仲内生真菌中抗苹果炭疽病活性菌株的筛选

Screening of endophytic fungi isolated from Eucommia ulmoides oliv. with antimicrobial activity against Colletotrichum gloeosporioides

对杜仲植株中的内生真菌进行分离纯化,共得到32株内生菌株。通过离体平板对峙培养检测、光学显微镜观察,以及测定在活体果实上的拮抗活性,研究杜仲拮抗真菌与病原菌及其寄主植物苹果果实之间的相互作用。对峙培养显示杜仲内生真菌DZGS07对苹果炭疽菌有较强拮抗作用,具有较强的营养和空间竞争能力,显微观察表明其能造成病原菌菌丝畸形等现象。在生防研究中,内生真菌DZGS07对苹果炭疽病具有较好的生防潜力,处理后的第7天防效达84.19%;对该菌株的ITS序列进行测定分析,初步鉴定DZGS07为炭疽菌属(Colletotrichum)的真菌。

Thirty-two endophytic fungi were isolated from the leaves, stems and fruits ofEucommia ulmoides oliv. The interaction between endophytic fungi and the phytopathogen or host apple fruit have been investigated by dual culture experiment on PDA plates and observed by light microscope, as well as antagonistic activity as- sessed in plant tests. Dual culture experiment indicated the strain DZGS07 had strong antagonistic activity on Colletotrichum gloeosporioides and high competition of space and nutrition. Microscopic observation proved the strain DZGS07 could cause mycelial malformation and so on. Furthermore, the strain DZGS07 had potential for biocontrol on Colletotrichum gloeosporioides, and the control efficacy was 84.19% against the disease after 7 days. Lastly, the strain DZGS07 was identified by molecular biology method, and the results showed the strain DZGS07 belongs to Colletotrichum.