气相色谱法检测牛奶及奶粉中共轭亚油酸

Determination of Conjugated Linoleic Acid in Milk and Milk Powder by Gas Chromatography

采用气相色谱对牛奶及奶粉中的共轭亚油酸的含量进行检测。样品通过脂肪提取、甲基化和上机测定,可以完全定性和定量分析CLA。结果表明:采用该方法可以使牛奶和奶粉中的c9,t11-CLA得到很好的分离,且方法简单、安全,结果准确可靠,检验数据重现性好。

A gas chromatographic （GC） method was developed and applied to determine conjugated linoleic acid （CLA） in milk and milk powder. Fat extraction from samples and methylation were made before GC analysis. Excellent separation of cg,tl 1-CLA was achieved. The developed method proved to be simple, safe, accurate, reliable and reproducible.