河南省周口地区瓜类白粉病的分子鉴定与分析

Molecular identification and analysis of cucurbits powdery mildew in Zhoukou District of Henan Province

对河南周口地区的瓜类白粉病进行了分子鉴定及分析.分别对黄瓜、甜瓜、葫芦、南瓜白粉病菌进行了扫描电子显微形态观察、核糖体DNA转录间隔区(ITS)克隆、测序及进化树分析.结果表明,周口地区的4种瓜类白粉病菌属于Sphaerotheca fuliginea,系统进化发现瓜类白粉病菌与豆类、美女樱、水母柱树白粉病菌种间差异小,同时发现该地区的瓜类白粉病菌与美洲、日本、澳大利亚、英国白粉病亲缘关系比较近,推测瓜类白粉病菌的进化与地域没有直接关系,而是与其自身的小种进化有关.

In this study,cucurbits powdery mildew in the Zhoukou region of Henan province were identified and analysed,the results provided the basis for pathogen defence and systematic evolution.In order to identify the pathogen,the characteristics of histological morphological,molecular identification of ribosomal DNA internal transcribed spacer （ITS） and phylogenetic trees were conducted.The results indicated that the cucurbits powdery mildew in Zhoukou belonged to Sphaerotheca fuliginea.Phylogenetic tree revealed that the differences between cucurbits powdery mildew and legumes,verbena hybrida,medusagyne oppositifolia powdery mildew were small,meanwhile the pathogen of Zhoukou was highly conserved with USA,Japan,Australia and UK.We speculated that the evolution of cucurbits powdery mildew may not be directly related to areas but to its own physiological race.