摘要
目的:探讨不同剂量氢气饱和生理盐水(HS)对烟雾吸入性损伤大鼠急性肺损伤的保护作用。方法:30只SD大鼠随机分为5组:对照组(C)、吸入性损伤组(I)、氢气饱和生理盐水低剂量组(2.5 mL/kg,HS L)、中剂量组(5 mL/kg,HS M)、高剂量组(10 mL/kg,HS H),24 h后留取标本,透射电镜观察肺组织病理变化,检测血清中肿瘤坏死因子α(TNF-α),肺组织匀浆的丙二醛(MDA)含量,免疫组织化学法测定肺组织核因子-κBp65(NF-κBp65)的表达,TUNEL检测肺组织凋亡情况。结果:中、高剂量氢气饱和生理盐水能明显减轻吸入性损伤大鼠的急性肺损伤,降低炎性因子(TNF-α)的释放,减轻组织氧化损伤(MDA低于致伤组),抑制肺组织NF-κBp65的活化及细胞凋亡,且HS H优于HS M,HS M、HSH组间差异有统计学意义(P<0.05),而HS L组效果不明显。结论:氢气饱和生理盐水可减轻吸入性损伤大鼠的急性肺损伤,且存在一定的量效关系。
Objective：To discuss the therapeutical effect on smoke inhalation injury in rats under different doses of hydrogen-rich saline.Methods：Thirty rats were randomly divided into 5 groups：control group （C）,inhalation group （Ⅰ）,low dose group （2.5 mL/kg,HSL）,medium dose group （5 mL/kg,HSM）,and high dose group （10 mL/kg,HSH）.At 24 hours after injured,all rats were sacrificed and specimens were taken.The histopathological changes of lung tissue were observed using electron micrography.Tumor necrosis factor-α （TNF-α） in plasma was quantified,malondialdehyde （MDA） content of lung tissue was also detected,the NF-κBp65 in lung speciments was examined by immunohistochemistry technology,and the apoptosis of lung was monitored by terminal deoxynucleotidyl transfercase dUTP nik end labeling （TUNEL） assay.Results：When a comparison was made with the inhalation group,hydrogen-rich saline treatment could significantly relieve acute lung injury induced by inhalation injury as indicated by ultrastructural histopathologic changes under electron micrography and decrease TNF-α level.Furthermore,hydrogen-rich saline could inhibit lipid oxidation （MDA level was significantly decreased）,the expression of NF-κBp65 and apoptosis of lung tissue,especially in medium and high dose group,while this change was not significant in low dose group.Conclusion：Hydrogen-rich saline treatment could significantly relieve acute lung injury induced by inhalation injury,with dose-effect relationship.
出处
《天津医科大学学报》
2013年第6期445-448,共4页
Journal of Tianjin Medical University
基金
天津市卫生局科技基金资助项目(2012KY05)
