脂多糖对牙周膜成纤维细胞IL-8和RANKL基因表达的调节作用

Regulation of IL-8 and RANKL gene expression by lipopolysaccharide in human periodontal ligament fibroblasts

目的:研究牙龈卟啉单胞菌(P.g.)脂多糖(LPS)对人牙周膜成纤维细胞(hPDLFs)中白细胞介素8(IL-8)和NF-κB受体活化因子配体(RANKL)基因表达的调节。方法:取第4代hPDLFs细胞在含有10%胎牛血清(FCS)的DMEM培养基中培养至完全融合后,分别用0.01、0.1、1、10、100 mg/L P.g.LPS作用hPDLFs 10 h,实时荧光定量PCR检测IL-8和RANKL mRNA的表达水平。结果:P.g.LPS浓度依赖性地刺激hPDLFs中IL-8和RANKL mRNA的表达水平升高,10 mg/L P.g.LPS上调IL-8 mRNA水平,达到峰值(P<0.01);100 mg/L P.g.LPS显著增强RANKL mRNA水平(P<0.01)。结论:高浓度的P.g.LPS明显刺激hPDLFs中IL-8和RANKL的基因表达增强。

Objective：To study the regulation of gene expression of interleukin 8 （IL-8） and receptor activator of NF-κB ligand （RANKL）by Porphyromonas gingivalis lipopolysaccharide （P.g.LPS） in human periodontal ligament fibroblasts （hPDLFs）.Methods：After the 4th generation hPDLFs cells were cultured in DMEM containing 10% fetal calf serum （FCS） to full fusion and hPDLFs were treated by 0.01,0.1,1,10,100 mg/L P.g.LPS was applied for 10 h and real-time PCR was performed to measure IL-8 and RANKL mRNA expression levels.Results：P.g.LPS dose-dependent stimulated IL-8 and RANKL mRNA expression in hPDLFs,10 mg/L P.g.LPS increased IL-8 mRNA levels to peak （P〈0.01）; RANKL mRNA levels were increased by 100 mg/L P.g.The result was statistically significantly （P〈0.01）.Conclusion：High concentration of P.g.LPS can stimulate IL-8 and RANKL gene expression increases significantly in hPDLFs.