艾灸对脑缺血再灌注损伤模型大鼠大脑皮质NF-κB p65蛋白表达的影响

Effects of moxibustion on cortical expression of NF-кB p65 protein in rats with cerebral ischemia-reperfusion injury

目的:观察艾灸对大脑皮质神经核因子κB p65(NF-κB p65)蛋白表达的影响,阐明艾灸治疗脑缺血再灌注损伤的作用机制。方法:雄性SD大鼠26只,分为假手术组(6只)、模型组(10只)和艾灸组(10只)。采用线栓法闭塞大脑中动脉2h后进行再灌注,制备局灶性脑缺血再灌注损伤大鼠模型。采用HE染色观察大鼠脑组织中炎性细胞浸润情况;放射免疫分析法测定大鼠血清白细胞介素-1β(IL-1β)和白细胞介素-2(IL-2)的含量;Western Blot技术检测大鼠大脑皮质NF-κB p65蛋白的表达。结果:与假手术组大鼠相比,模型组大鼠患侧脑组织中可见大量炎性细胞浸润、血清IL-1β和IL-2含量增多,大脑皮质NF-κB p65蛋白表达量增加(P<0.05);而艾灸组大鼠患侧脑组织中炎性细胞减少、血清IL-1β和IL-2含量降低(P<0.05)、大脑皮质NF-κB p65蛋白表达减少(P<0.05)。结论:减少大脑皮质NF-κB p65蛋白表达可能是艾灸治疗大鼠脑缺血再灌注损伤的作用机制之一。

Objective： To observe the effects of moxibustion on cortical expression of nuclear factor -kB（NF-kB） p65 protein in rats with cerebral ischemia-reperfusion injury, and to clarify the possible mechanism of moxibustion for treatment of cerebral ischemia reperfusion injury. Methods： Twenty-six male SD rats were randomly divided into three groups, sham group （n=6）, model group （n=10） and moxibustion group （n=10）. Focal cerebral ischemia-reperfusion injury model was induced for 2h by middle cerebral artery occlusion （MCAO） followed by reperfusion. HE staining was used to examine inflammatory cells in the brain, radioimmunoassay was used to calculate the concentration of interleukin-1β and interleukin-2 in the serum, and western blot was used to detect the expression of NF-kB p65 protein in the cerebral cortex, Results： Compared with sham group, a large number of inflammatory cells existed in the rat brain of model group, the serum concentrations of interleukin- 1β and interleukin-2 were increased （P〈0.05）, and the cortical expression of NF-kB p65 protein were risen （P〈0.05）. Less inflammatory cells existed in the rat brain of moxibustion group, the serum concentrations of interleukin-1β and interleukin-2 were decreased, and the cortical expression of NF-KB p65 protein was reduced （P〈0.05）. Conclusion： Reducing expression of NF-KB p65 protein in cerebral cortex might be one of mechanisms of moxibustion attenuating focal cerebral ischemia-reperfusion injury.