益气消癥法对雌激素诱导子宫肌瘤豚鼠子宫组织ERα信号转导通路相关蛋白表达的影响

Influence of Yiqi Xiaozheng method on the direction of the factor about signal transduction pathway based on the estrogen receptor

目的:研究益气消癥法对雌激素诱导子宫肌瘤模型豚鼠子宫组织基于ERα的信号转导通路的影响。方法:通过去势+皮下注射苯甲酸雌二醇建立子宫肌瘤模型,通过免疫组化法检测各组子宫组织ERα、细胞外信号调节激酶2(MEK2)、磷酸化细胞外信号调节激酶(p-ERK)蛋白表达。并对以上指标进行相关性分析。结果:模型组豚鼠子宫肌组织ERα、MEK2、p-ERK蛋白表达显著高于正常组(P<0.01),中药各剂量组均有不同程度的降低,其中中药高剂量组豚鼠子宫组织ERα、MEK2、p-ERK蛋白表达显著低于模型组及西药组(P<0.01),且ERα与MEK2及p-ERK均有显著相关性(P<0.01)。结论:益气消癥法可能是通过干预ERα信号通路抑制肌瘤细胞增殖,从而达到治疗子宫肌瘤的目的。

Objective： To study the effects of Yiqi Xiaozheng method on the direction of the factor about signal transduction pathway which is based on the estrogen receptor a. Methods： The uterine leiomyoma model was established in guinea pigs by removing the ovaries and injecting the estradiol benzoate in abdominal, and then the protein expression levels of ERa, MEK2 and p-ERK of guinea pigs＇ uterine tissues were dectected in each group by immunohistochemistry staining. Furthermore the above data was analysed. Results： The expression levels of ERa, MEK2, p-ERK in modle group were higher than the normal group （P〈0.01）. Guinea pigs in high dose group of traditional Chinese medicine have lower expression level of ERa, MEK2 and p-ERK in uterine tissue than model group and the western medicine group （P〈0.01）. There were significant positive correlation between ERa and MEK, p-ERK （P〈0.01）. Conclusion： Yiqi Xiaozheng method can obviously reduce the protein expression level of MEK-2 and p-ERK in uterine tissue, which are signal transduction associated protein. This may be the mechanism of Yiqi Xiaozheng Method in treating uterine leiomyoma.