朝医加味麻黄定喘汤在小鼠哮喘模型中对ERK信号通路的影响

Protective effects and mechanism of Jiawei Mahuang Dingchuan Decoction on cough variant asthma mice

目的:探讨加味麻黄定喘汤对哮喘小鼠气道炎症和Thl/Th2类细胞因子变化的影响及其可能机制。方法:雄性BALB/c小鼠32只随机分成4组,即正常组、模型组和加味麻黄定喘汤低、高剂量组。分别采用酶联免疫吸附法(ELISA)和蛋白质印迹检测支气管肺泡灌洗液(BALF)中白细胞介素-4(IL-4)、IL-5、IL-13及γ-干扰素(IFN-γ)含量,并观察BALF中炎症细胞和肺组织病理学改变。免疫组化法检测细胞外信号调节蛋白激酶(ERK)在肺内表达。结果:模型组小鼠BALF中炎症细胞计数、IL-4、IL-5、IL-13水平升高,IFN-γ水平降低,与正常组比较,差异有统计学意义(P<0.05)。加味麻黄定喘汤低、高剂量组小鼠BALF中炎症细胞计数、IL-4、IL-5、IL-13水平降低,IFN-γ水平明显上升,与模型组比较,差异有统计学意义(P<0.05)。加味麻黄定喘汤能明显减轻哮喘小鼠肺组织病理学改变,加味麻黄定喘汤低、高剂量组之间比较,有显著性差异(P<0.05)。模型组ERK在肺内表达较正常组增强,加味麻黄定喘汤低、高剂量组肺组织中ERK的蛋白质印迹表达均较模型组明显下降(P<0.05)。结论:加味麻黄定喘汤对哮喘小鼠具有保护作用,其机制可能与抑制ERK磷酸化,调节IL-4/IFN-γ平衡失调、减轻炎症细胞浸润有关。

Objective： To explore the protective effects and mechanism of ethanol extract of Jiawei Mahuang Dingchuan Decoction injection on asthmatic mouse. Methods： OVA was injected intraperitoneally and inhaled to produce the asthmatic model. Thirty two mouse were randomly divided into four groups： control group, asthma group and Decoction injection groups of high and low dose. The concentrations of IL-4, IL-5, IL-13 and IFN-γ in BALF, the phosphor-ERK in lung tissueswere respectively measured by ELISA. The number of inflammatory cells in BALF and histropathology changes were observed. Results： In asthmatic group, the number of inflammatory cells and the concentrations of IL-4, IL-5, IL-13 in BALF and phosphor- ERK in lung tissue we re higher, but IFN-γ were lower than those in normal control mouse （P〈0.05）. In Decoction injection group, the number of inflammatory cells, the concentrations of IL-4, IL-5, IL-13 in BALF （P〈0.05）, and histropathology damage was alleviated significantly. The efficacies in the Decoction injection groups of high and low dose were similar. Conclusion： Decoction injection effectively treats asthma by inhibiting the express ion of phosphor-ERK, correcting the inbalance of IFN-γ/IL-4 and decreasing the number of inflammatory cells.